Microbiology with ultra-high-throughput sequencing

We are using the Illumina high-throughput sequencing platform to sequence whole microbial genomes and transcriptomes. This technology generates hundreds of millions of short reads per run, reaching multiple giga bases of sequence per day. The Illumina technology enables us to study the evolution of bacteria, discover phenotype altering mutations, and document dynamic evolutionary processes.

Bacterial whole genome evolution

Using high throughput sequencing technologies, we studied the transfer of genetic material between bacetria (Sorek et al, Science 2007), documented processes of genome shrinkage in bacteria (Moran et al, Science 2009), and found how bacterial genomes evolve in response to phage attacks (Avrani et al, Nature 2011). We also develop methods for accurate detection of individual mutations in genomes using short-read sequencing technologies (Wurtzel et al, 2010).

Illumina HiSeq 2000~