Anne NICOLAS 
Born January 15, 1961, in Tunis, Tunisia 
French citizen 
Single 
 

Weizmann Institute of Science  Neurobiology Department  76100 Rehovot, Israel.  E-mail: anne.nicolas@weizmann.ac.il  Fax: +972 (0)8 934 41 54 Phone: +972 (0)8 934 24 79

Personal address:  Gordon Street 43 C / 14  Rehovot 76100  Israel  Phone: +972 (0)8 947 33 41

Neurosciences, structure-function relationship, macromolecular crystallography

2001-present Postdoctoral fellow

STRUCTURAL BIOLOGY and Department, Weizmann Institute of Science, Israel
Directed by Prof. Z. Shakked
Structural Studies of DNA
 

1998-2000 Postdoctoral fellow

STRUCTURAL BIOLOGY and NEUROBIOLOGY Departments, Weizmann Institute of Science, Israel
Directed by Prof. J. L. Sussman and Prof. I. Silman. Under technical supervision of L. Toker
Used biochemical and biophysical tools to purify and characterize acetylcholinesterases from Torpedo californica and Bungarus fasciatus.
 

1997-98 Postdoctoral fellow

LABORATORY OF  CRYSTAL AND STRUCTURAL CHEMISTRY, Utrecht University, The Netherlands
Under supervision of Prof. J. Kroon
Participated in Laue diffraction studies on acetylcholinesterase crystals, with the ultimate goal of time-resolved studies, in collaboration with the Laboratory of Bioorganic Chemistry, Strasbourg and the ESRF White Beamline ID9 group, Grenoble.

Obtained four crystal forms of Torpedo californica acetylcholinesterase
 

1992-96 Ph.D. student

LABORATORY OF BIOLOGICAL MACROMOLECULE ARCHITECTURE AND FUNCTION, CNRS, Marseille, France
Directed by Dr. C. Cambillau. Under supervision of Dr. C. Withers-Martinez
Obtained crystal and resolved structures of ten cutinase mutants, an inhibitor-cutinase complex and cutinase expressed in Saccharomyces cerevisiae and in Aspergillus niger

Collected and refined crystal data of cutinase expressed in E. coli at 1.15 Å with conventional X-ray source and collected crystal data at 1.05 Å resolution with synchrotron X-ray source at DESY (Hamburg, Germany) and ESRF (Grenoble, France)

Compared 18 alpha/beta hydrolase fold structures in their native and inhibited forms 

LABORATORY OF ENZYMATIC LIPOLYSE, CNRS, Marseille, France
Directed by Dr. R. Verger. Under supervision of Dr. C. Cudrey and F. Ferrato
Determined by spectrometry and titrametry methods lipase activity of cutinase mutants and inhibition curves of cutinase in presence of different covalent inhibitors
 

1991-92 Pre-doctoral Trainee

LABORATORY OF BIOLOGICAL MACROMOLECULE ARCHITECTURE AND FUNCTION, CNRS, Marseille, France
Directed by Dr. C. Cambillau. Under supervision of Dr. C. Withers-Martinez
Obtained crystal and resolved structures of a cutinase mutant 
Determined kinetic parameters of two inhibitor-cutinase complexes and crystallised them
 

1989-91 M.S. Trainee

LABORATORY OF NEUROENDOCRINOLOGY, INSERM, Marseille, France
Directed by Prof. C. Oliver. Under supervison of Dr. P. Giraud
Helped find optimal conditions for rat nervous cell culture with an artificial gene (preparation of striatum rat neural cell culture, plasmid amplification and purification, neural cell transfection, CAT activity measurements)
 

LABORATORY OF CHILD PSYCHOLOGY , Institut des Hautes Etudes, Paris, France
Directed by Dr. H. Bloch
Participated in research on sensorial perception in the human fetus

LABORATORY OF HUMAN NEUROBIOLOGY, CNRS, Marseille, France
Directed by Dr. J. C. Gilhodes
Participated in research on sensori-motor coordination based on multi-sensorial inputs and neural networks

LABORATORY OF HIGH PRESSURE PHYSIOLOGY, CNRS, Marseille, France
Directed by Dr. J. Rostain
Participated in research on electrophysiological studies on rat caudate-putamen and hippocampus CA1 cells under high pressure

Tango instructor, sailing, hiking, diving

   Prof. Jan Kroon j.kroon@chem.uu.nl

   Dr. Christian Cambillau chris@afmb.cnrs-mrs.fr

   Prof. Joel L. Sussman Joel.Sussman@weizmann.ac.il