The following services are provided at the ISPC facility
Cloning/Mutagenesis & Expression Screening
- Cloning/mutagenesis (PCR, digestion, cloning, transformation & sequencing)
- Small scale expression test in E. coli
- Expression test in P. pastoris
- Viral production and expression test in insect cells
- Expression test in HEK293 cells
Large Scale Cultures for Protein Production
- 5L culture - E. coli
- 5L culture – P. pastoris
- 1.5L culture – Insect cells
- 1.5L culture – HEK293 cells
Protein Purification & Refolding
- Protein extraction from cells and tissues
- Protein purification (single and multiple chromatographic steps)
- Removal of tags
- Method development
- Refolding of inclusion bodies
- Protein characterization
Crystallization & Structure Determination
- Crystallization screening utilizing sitting and hanging drop vapor diffusion
- Crystallization screening utilizing the Lipidic Cubic Phase for membrane proteins
- Optimization of crystallization conditions
- X-ray data collection
- Structure determination and structure analysis
For Weizmann users - please use THIS LINK to order our services at Internal Services.