The immune system is composed of large repertoire of B cells, the precursors of antibody forming cells. Each express a unique antibody with different affinity and specificity. We examine how selection of B cells from the pre-vaccinated repertoire are selected for entry into the antibody immune response for differentiation into germinal center cells as well as antibody forming cells. For this we use multiple approaches wherein we examine interactions and niche formation of the immune cells by light sheet fluorescence microscopy and intravital two photon microscopy. Using these methods, we visualize and study the antibody immune response in a whole lymphoid organ at the single cell resolution and visualize entry and exit of B cells from the antibody immune response .