Visualization of soluble receptors and binding proteins by polyacrylamide gel electrophoresis (PAGE)

Discovery of soluble cytokine Receptors and Binding Proteins

Throughout the years we isolated and characterized six novel soluble receptors (TNF receptors – the TBPI and  TBPII, IL-6R, Interferon-γR, LDLR, Interferon α/βR) and three unexpected binding proteins, the IL-18BP, the IL-32BP/PR3 and the heparanase binding protein.

Proteins translated to drugs

From Bench to Bedside

Interferon-β was developed as a drug known as Rebif™, which is used for the treatment of multiple sclerosis. One of the soluble TNF receptors was translated to a drug known as Enbrel™ and is used by rheumatoid arthritis patients. IL-18BP was used as a compasionate drug and saved the life of a baby. At present it is in phase II and III clinical studies.

Ligand affinity chromatography and Mass Spectrometry

A unique approach (methods used)

My approach of combining an enriched source of proteins (500-1000 fold concentrated normal human urine) together with highly specific isolation method, the ligand affinity chromatography, enabled a rapid and efficient isolation of not only soluble receptors corresponding to cell-associated receptors, but also independent binding proteins and associated enzymes.