Regulation of gene expression at the transcriptional and translational levels is fundamental to all biological activities and is frequently altered in disease states. Our broad research interests are to elucidate the means by which regulatory information encoded within the genes is decoded during transcription and translation, to reveal the connections between the two processes and to develop tools to manipulate them. To this end our aims are: (i) to understand mechanistic aspects of transcription, focusing on the initiation and elongation stages; (ii) to unravel the molecular basis of translation mechanisms that are linked to transcription; (iii) to search for drugs that target specific components of the transcription machinery for the teratment of cancer and chronic inflammation. We address these issues utilizing several biological systems:
- NF-κB, the major pro-inflammatory transcription factor
- TISU, a dual transcription and translation regulatory element
- The core promoter, a central regulatory element in transcription initiation
Our long term goal is to achieve deeper understanding of various stages of gene expression with an emphasis on their crosstalk and their involvement in diseases.