Bacterial Chemotoxis

  1. Starbird C. A., Tomasiak T. M., Singh P. K., Yankovskaya V., Maklashina E., Eisenbach M., Cecchini G. & Iverson T. M. (2018) New crystal forms of the integral membrane Escherichia coli quinol: fumarate reductase suggest that ligands control domain movement. Journal of Structural Biology, 202(1):100-104
  2. Baron S., Afanzar O. & Eisenbach M. (2017) Methylation-independent adaptation in chemotaxis of Escherichia coli involves acetylation-dependent speed adaptation. FEBS Letters, 591(2):331-337
  3. Maklashina E., Rajagukguk S., Starbird C. A., Mcdonald W. H., Koganitsky A., Eisenbach M., Iverson T. M. & Cecchini G. (2016) Binding of the Covalent Flavin Assembly Factor to the Flavoprotein Subunit of Complex II. Journal of Biological Chemistry, 291(6):2904-2916
  4. Fraiberg M., Afanzar O., Cassidy C. K., Gabashvili A., Schulten K., Levin Y. & Eisenbach M. (2015) CheY's acetylation sites responsible for generating clockwise flagellar rotation in Escherichia coli. Molecular Microbiology, 95(2):231-244
  5. Perez Cerezales C. S., Boryshpolets S., Afanzar O., Brandis A., Nevo R., Kiss V. & Eisenbach M. (2015) Involvement of opsinsin mammalian sperm thermotaxis. Scientific Reports, 5:16146-16146
  6. Armon L., Ben-Ami I., Ron-El R. & Eisenbach M. (2014) Human oocyte-derived sperm chemoattractant is a hydrophobic molecule associated with a carrier protein. Fertility and Sterility, 102(3):885-890
  7. Caplan S. R., Zarbiv G., Li H., Wolf A., Cecchini G., Sourjik V. & Eisenbach M. (2013) Are "Power-Packs" Associated with the Switch-Motor Complex of Bacterial Flagella?. Biophysical Journal, 104(2):641A-641A
  8. Bahat A., Caplan S. R. & Eisenbach M. (2012) Thermotaxis of Human Sperm Cells in Extraordinarily Shallow Temperature Gradients Over a Wide Range. PLoS One, 7(7):
  9. Zarbiv G., Li H., Wolf A., Cecchini G., Caplan S. R., Sourjik V. & Eisenbach M. (2012) Energy Complexes Are Apparently Associated with the Switch-Motor Complex of Bacterial Flagella. Journal of Molecular Biology, 416(2):192-207
  10. Armon L., Caplan S. R., Eisenbach M. & Friedrich B. M. (2012) Testing Human Sperm Chemotaxis: How to Detect Biased Motion in Population Assays. PLoS One, 7(3):
  11. Armon L. & Eisenbach M. (2011) Behavioral Mechanism during Human Sperm Chemotaxis: Involvement of Hyperactivation. PLoS One, 6(12):e28359
  12. Liarzi O., Barak R., Bronner V., Dines M., Sagi Y., Shainskaya A. & Eisenbach M. (2010) Acetylation represses the binding of CheY to its target proteins. Molecular Microbiology, 76(4):932-943
  13. Gakamsky A., Armon L. & Eisenbach M. (2009) Behavioral response of human spermatozoa to a concentration jump of chemoattractants or intracellular cyclic nucleotides. Human Reproduction, 24(5):1152-1163
  14. Oren-Benaroya R., Orvieto R., Gakamsky A., Pinchasov M. & Eisenbach M. (2008) The sperm chemoattractant secreted from human cumulus cells is progesterone. Human Reproduction, 23(10):2339-2345
  15. Bahat A. & Eisenbach M. (2008) Human sperm thermotaxis is mediated by phospholipase C and inositol trisphosphate receptor. Biology of Reproduction76-76
  16. Cohen-Ben-Lulu G. N., Francis N. R., Shimoni E., Noy D., Davidov Y., Prasad K., Sagi Y., Cecchini G., Johnstone R. M. & Eisenbach M. (2008) The bacterial flagellar switch complex is getting more complex. EMBO Journal, 27(7):1134-1144
  17. Benaroya R. O., Kipnis J. & Eisenbach M. (2007) Pragocytosis of human post-capacitated spermatozoa by macrophages. Biology of Reproduction123-123
  18. Eisenbach M. (2006) Behavioral responses of bacteria to chemostimuli. Chemical Senses, 31(8):E41-E41
  19. Tur-Kaspa I., Goldberg-Bittman L., Bahat A. & Eisenbach M. (2006) Natural sperm separation technique by thermotaxis for ART. Human Reproduction, 21:I212-I213
  20. Sun F., Bahat A., Gakamsky A., Girsh E., Katz N., Giojalas L., Tur-Kaspa I. & Eisenbach M. (2005) Human sperm chemotaxis: both the oocyte and its surrounding cumulus cells secrete sperm chemoattractants. Human Reproduction, 20(3):761-767
  21. Barak R., Prasad K., Shainskaya A., Wolfe A. & Eisenbach M. (2004) Acetylation of the chemicals response regulator CheY by acetyl-CoA synthetase purified from Escherichia coli. Journal of Molecular Biology, 342(2):383-401
  22. Giojalas L., Rovasio R., Fabro G., Gakamsky A. & Eisenbach M. (2004) Timing of sperm capacitation appears to be programmed according to egg availability in the female genital tract. Fertility and Sterility, 82(1):247-249
  23. Bahat A., Tur-Kaspa I., Gakamsky A., Giojalas L., Breitbart H. & Eisenbach M. (2003) Thermotaxis of mammalian sperm cells: A potential navigation mechanism in the female genital tract. Nature Medicine, 9(2):149-150
  24. Fabro G., Rovasio R., Civalero S., Frenkel A., Caplan S., Eisenbach M. & Giojalas L. (2002) Chemotaxis of capacitated rabbit spermatozoa to follicular fluid revealed by a novel directionality-based assay. Biology of Reproduction, 67(5):1565-1571
  25. Barak R. & Eisenbach M. (1999) Chemotactic-like response of Escherichia coli cells lacking the known chemotaxis machinery but containing overexpressed CheY. Molecular Microbiology, 31(4):1125-1137
  26. McEvoy M., Bren A., Eisenbach M. & Dahlquist F. (1999) Identification of the binding interfaces on CheY for two of its targets, the phosphatase CheZ and the flagellar switch protein FliM. Journal of Molecular Biology, 289(5):1423-1433
  27. Jaiswal B., Eisenbach M. & Tur-Kaspa I. (1998) Which inducers and probes should be used for testing partial or complete acrosome reaction in human spermatozoa?. Human Reproduction, 13:75-76
  28. Barak R., Abouhamad W. & Eisenbach M. (1998) Both acetate kinase and acetyl coenzyme A synthetase are involved in acetate-stimulated change in the direction of flagellar rotation in Escherichia coli. Journal of Bacteriology, 180(4):985-988
  29. Montrone M., Eisenbach M., Oesterhelt D. & Marwan W. (1998) Regulation of switching frequency and bias of the bacterial flagellar motor by CheY and fumarate. Journal of Bacteriology, 180(13):3375-3380
  30. Prasad K., Caplan S. & Eisenbach M. (1998) Fumarate modulates bacterial flagellar rotation by lowering the free energy difference between the clockwise and counterclockwise states of the motor. Journal of Molecular Biology, 280(5):821-828
  31. Jaiswal B., Cohen-Dayag A., Tur-Kaspa I. & Eisenbach M. (1998) Sperm capacitation is, after all, a prerequisite for both partial and complete acrosome reaction. FEBS Letters, 427(2):309-313
  32. Blat Y. & Eisenbach M. (1996) Conserved C-terminus of the phosphatase CheZ is a binding domain for the chemotactic response regulator CheY. Biochemistry, 35(18):5679-5683
  33. Blat Y. & Eisenbach M. (1996) Mutants with defective phosphatase activity show no phosphorylation-dependent oligomerization of CheZ - The phosphatase of bacterial chemotaxis. Journal of Biological Chemistry, 271(2):1232-1236
  34. Blat Y. & Eisenbach M. (1996) Oligomerization of the phosphatase CheZ upon interaction with the phosphorylated form of CheY - The signal protein of bacterial chemotaxis. Journal of Biological Chemistry, 271(2):1226-1231
  35. Blat Y. & Eisenbach M. (1995) Tar-Dependent and Tar-Independent Pattern-Formation by Salmonella-Typhimurium. Journal of Bacteriology, 177(7):1683-1691
  36. Karaivanov M., Eisenbach M. & Caplan S. (1995) Fluctuations in Rotation Rate of the Flagellar Motor of Escherichia-Coli. Biophysical Journal, 69(1):250-263
  37. Kumari S., Tishel R., Eisenbach M. & Wolfe A. (1995) Cloning, Characterization, and Functional Expression of Acs, the Gene Which Encodes Acetyl-Coenzyme-a Synthetase in Escherichia-Coli. Journal of Bacteriology, 177(10):2878-2886
  38. Cohendayag A., Turkaspa I., Dor J., Mashiach S. & Eisenbach M. (1995) Sperm capacitation in human is transient and correlates with chemotactic responsiveness. Human Sperm Acrosome Reaction, 236:397-398
  39. Cohendayag A. & Eisenbach M. (1994) Potential Assays For Sperm Capacitation in Mammals. American Journal Of Physiology-Cell Physiology, 267(5):C1167-C1176
  40. Cohendayag A., Ralt D., Turkaspa I., Manor M., Makler A., Dor J., Mashiach S. & Eisenbach M. (1994) Sequential Acquisition of Chemotactic Responsiveness by Human Spermatozoa. Biology of Reproduction, 50(4):786-790
  41. Ralt D., Manor M., Cohendayag A., Turkaspa I., Benshlomo I., Makler A., Yuli I., Dor J., Blumberg S., Mashiach S. & Eisenbach M. (1994) Chemotaxis and Chemokinesis of Human Spermatozoa to Follicular Factors. Biology of Reproduction, 50(4):774-785
  42. Margolin Y., Barak R. & Eisenbach M. (1994) Arsenate Arrests Flagellar Rotation in Cytoplasm-Free Envelopes of Bacteria. Journal of Bacteriology, 176(17):5547-5549
  43. Welch M., Oosawa K., Aizawa S. & Eisenbach M. (1994) Effects of Phosphorylation, Mg2+, and Conformation of the Chemotaxis Protein CheY on Its Binding to the Flagellar Switch Protein Flim. Biochemistry, 33(34):10470-10476
  44. Eisenbach M. & Turkaspa I. (1994) Human Sperm Chemotaxis is Not Enigmatic Anymore. Fertility and Sterility, 62(2):233-235
  45. Blat Y. & Eisenbach M. (1994) Phosphorylation-Dependent Binding of the Chemotaxis Signal Molecule CheY to Its Phosphatase, Chez. Biochemistry, 33(4):902-906
  46. Barak R., Welch M., Yanovsky A., Oosawa K. & Eisenbach M. (1992) Acetyladenylate or Its Derivative Acetylates the Chemotaxis Protein CheY Invitro and Increases Its Activity at the Flagellar Switch. Biochemistry, 31(41):10099-10107
  47. Barak R. & Eisenbach M. (1992) Correlation Between Phosphorylation of the Chemotaxis Protein-CheY and Its Activity at the Flagellar Motor. Biochemistry, 31(6):1821-1826
  48. Barak R. & Eisenbach M. (1992) Fumarate or a Fumarate Metabolite Restores Switching Ability to Rotating Flagella of Bacterial Envelopes. Journal of Bacteriology, 174(2):643-645
  49. Rosenbluh A. & Eisenbach M. (1992) Effect of Mechanical Removal of Pili on Gliding Motility of Myxococcus-Xanthus. Journal of Bacteriology, 174(16):5406-5413
  50. Eisenbach M. & Ralt D. (1992) Precontact Mammalian Sperm Egg Communication and Role in Fertilization. American Journal of Physiology, 262(5):C1095-C1101
  51. Eisenbach M., Barak R. & Ravid S. (1990) Restoration of Signal Transduction in Bacterial Chemotaxis. Biological Chemistry Hoppe-Seyler, 371(10):898-898
  52. Eisenbach M., Constantinou C., Aloni H. & Shinitzky M. (1990) Repellents For Escherichia-Coli Operate Neither by Changing Membrane Fluidity Nor by Being Sensed by Periplasmic Receptors During Chemotaxis. Journal of Bacteriology, 172(9):5218-5224
  53. Eisenbach M., Wolf A., Welch M., Caplan S., Lapidus I., Macnab R., Aloni H. & Asher O. (1990) Pausing, Switching and Speed Fluctuation of the Bacterial Flagellar Motor and Their Relation to Motility and Chemotaxis. Journal of Molecular Biology, 211(3):551-563
  54. Eisenbach M. & Matsumura P. (1988) Invitro Approach to Bacterial Chemotaxis. Botanica Acta, 101(2):105-110
  55. Lapidus I., Welch M. & Eisenbach M. (1988) Pausing of Flagellar Rotation is a Component of Bacterial Motility and Chemotaxis. Journal of Bacteriology, 170(8):3627-3632
  56. Margolin Y. & Eisenbach M. (1984) Voltage Clamp Effects on Bacterial Chemotaxis. Journal of Bacteriology, 159(2):605-610
  57. Eisenbach M., Margolin Y., Ciobotariu A. & Rottenberg H. (1984) Distinction Between Changes in Membrane-Potential and Surface-Charge Upon Chemotactic Stimulation of Escherichia-Coli. Biophysical Journal, 45(2):463-467
  58. Ravid S. & Eisenbach M. (1984) Direction of Flagellar Rotation in Bacterial-Cell Envelopes. Journal of Bacteriology, 158(1):222-230
  59. Ravid S. & Eisenbach M. (1984) Minimal Requirements For Rotation of Bacterial Flagella. Journal of Bacteriology, 158(3):1208-1210
  60. Lelkes P., Klein L., Marikovsky Y. & Eisenbach M. (1984) Liposome-Mediated Transfer of Macromolecules Into Flagellated Cell Envelopes from Bacteria. Biochemistry, 23(3):563-568
  61. Eisenbach M., Raz T. & Ciobotariu A. (1983) A Process Related to Membrane-Potential Involved in Bacterial Chemotaxis to Galactose. Biochemistry, 22(13):3293-3298
  62. Ravid S. & Eisenbach M. (1983) Correlation Between Bacteriophage-Chi Adsorption and Mode of Flagellar Rotation of Escherichia-Coli Chemotaxis Mutants. Journal of Bacteriology, 154(2):604-611
  63. Ravid S. & Eisenbach M. (1983) Bacteriophage Chi Adsorption and the Mode of Flagellar Rotation in Behavioral Mutants of Escherichia-Coli. Israel Medical Association Journal, 19(7):685-685
  64. Eisenbach M., Klein L. & Lelkes P. (1983) Liposome-Mediated Transfer of Macromolecules Into Flagellated Cell Envelopes from Escherichia-Coli. Biophysical Journal, 41(2):A359-A359
  65. Corcia A., Eisenbach M., Wilchek M. & Caplan S. (1979) Increased Difference in Surface-Charge For Improving the Electrical Orientation of Gel-Entrapped Purple-Membrane Fragments. Israel Medical Association Journal, 15(1):110-110
  66. Eisenbach L. & Eisenbach M. (1979) Electrophoretic Mobility of Membrane Fragments on a Sucrose Gradient - Application to Isolated Purple Membrane Fragments from Halobacterium-Halobium. Analytical Biochemistry, 92(1):228-232
  67. Eisenbach M., Garty H., Bakker E., Klemperer G., Rottenberg H. & Caplan S. (1978) Kinetic-Analysis of Light-Induced ph Changes in Bacteriorhodopsin-Containing Particles from Halobacterium-Halobium. Biochemistry, 17(22):4691-4698
  68. Garty H., Eisenbach M., Rottenberg H. & Caplan S. (1977) Kinetics of Light-Induced ph Changes in Halobacterium-Halobium Suspensions. Israel Medical Association Journal, 13(9):951-951
  69. Cooper S., Garty H., Eisenbach M., Johnstone R., Rottenberg H. & Caplan S. (1977) Na+-h+ Exchange System in Sub-Bacterial Particles of Halobacterium-Halobium. Israel Medical Association Journal, 13(9):951-952
  70. Eisenbach M., Weissmann C., Tanny G. & Caplan S. (1977) Bacteriorhodopsin-Loaded Charged Synthetic Membranes - Utilization of Light Energy to Generate Electrical Current. FEBS Letters, 81(1):77-80
  71. Garty H., Klemperer G., Eisenbach M. & Caplan S. (1977) Direction of Light-Induced ph Changes in Purple Membrane Suspensions - Influence of ph and Temperature. FEBS Letters, 81(2):238-242
  72. Eisenbach M., Bakker E., Korenstein R. & Caplan S. (1976) Bacteriorhodopsin - Biphasic Kinetics of Phototransients and of Light-Induced Proton-Transfer by Sub-Bacterial Halobacterium-Halobium Particles and by Reconstituted Liposomes. FEBS Letters, 71(2):228-232