I am a Senior Staff Scientist at the Electron Microscopy Unit at the Weizmann Institute of Science.
I specialize in cryo-preparation techniques of aqueous samples, e.g. tissues, cells, organelles, gels, liposome suspensions etc. for electron microscopy (EM). By very fast freezing, sometimes under very high pressure, these techniques allow for the preservation of samples in a state, which is close to their native hydrated state, albeit in a solid rather than in a liquid phase. Thus, the ultrastructure observed in the microscope may faithfully reflect the structure of the native sample.
Following freezing, the samples can be visualized by various imaging techniques, such as transmission electron microscopy (TEM), cryo scanning electron microscopy (cryo-SEM), transmission or scanning-transmission electron tomography (CTET/CSTET).