Fluorescence microscopy is one of the most versatile imaging method in biology. However, the resolution of a conventional wide-field fluorescence microscope is limited by the diffraction of light to several hundred nanometers. Stochastic optical reconstruction microscopy (STORM) is a single molecule based super resolution light microscopy method. In this method, photoswitchable fluorescent probes are used to separate the spatially overlapping images of individual molecules, allowing to determine their position in high precision and reconstruct a high resolution three-dimensional multicolor fluorescence image.
STORM imaging of microtubules in amoebae.
In collaboration with Liran Ben-Yaacob and Prof. Avi Minsky from the Weizmann Institute of Science