We established a single-molecule system for mapping combinatorial chromatin modifications, and a single-cell platform to analyze a wide panel of histone modifications and chromatin regulators. We apply these powerful methods to address basic questions in epigenetic regulation of various cancer models.
The cell-free DNA (cfDNA) that circulates in the plasma and serum of humans mostly originates from apoptosis of normal hematopoietic cells. In some physiological conditions or diseases, cfDNA may originate from a different distribution of tissues, a phenomenon that is being exploited for clinical applications. We establish methodologies to identify the tissue contribution of plasma circulating nucleosomes under different physiological conditions that leverage the unique properties of the single-molecule chromatin profiling technology