All living cells utilize conserved systems responsible for membrane protein biogenesis, including the signal recognition particle (SRP) and its receptor.
The SRP system contains a membrane-bound SRP-receptor and an SRP protein-RNA complex, which recognizes nascent hydrophobic peptides in the process of translation. Whereas the SRP pathway has been elucidated mainly through a remarkable series of in vitro studies, we study major aspects of the pathway in vivo.
Our results were both surprising and unpredicted. Together, these studies support an alternative order of events in the E. coli targeting pathway. Our model predicts that (i) The SRP receptor is able to deliver ribosomes to the membrane during its own translation; (ii) mRNAs of membrane proteins might be targeted to membrane associated ribosomes, independently of translation; and (iii) the SRP functions downstream of the SRP-receptor. This hypothesis is currently being further investigated in our lab.