Bacterial Chemotoxis

Afanzar O., Di Paolo D., Eisenstein M., Levi K., Plochowietz A., Kapanidis A. N., Berry R. M. & Eisenbach M. (2021) The switching mechanism of the bacterial rotary motor combines tight regulation with inherent flexibility. EMBO Journal, 40(6):104683
Koganitsky A., Tworowski D., Dadosh T., Cecchini G. & Eisenbach M. (2019) A Mechanism of Modulating the Direction of Flagellar Rotation in Bacteria by Fumarate and Fumarate Reductase. Journal of Molecular Biology, 431(19):3662-3676
Starbird C. A., Tomasiak T. M., Singh P. K., Yankovskaya V., Maklashina E., Eisenbach M., Cecchini G. & Iverson T. M. (2018) New crystal forms of the integral membrane Escherichia coli quinol: fumarate reductase suggest that ligands control domain movement. Journal of Structural Biology, 202(1):100-104
Baron S. & Eisenbach M. (2017) CheY acetylation is required for ordinary adaptation time in Escherichia coli chemotaxis. FEBS Letters, 591(13):1958-1965
Baron S., Afanzar O. & Eisenbach M. (2017) Methylation-independent adaptation in chemotaxis of Escherichia coli involves acetylation-dependent speed adaptation. FEBS Letters, 591(2):331-337
Maklashina E., Rajagukguk S., Starbird C. A., Mcdonald W. H., Koganitsky A., Eisenbach M., Iverson T. M. & Cecchini G. (2016) Binding of the Covalent Flavin Assembly Factor to the Flavoprotein Subunit of Complex II. Journal of Biological Chemistry, 291(6):2904-2916
Fraiberg M., Afanzar O., Cassidy C. K., Gabashvili A., Schulten K., Levin Y. & Eisenbach M. (2015) CheY's acetylation sites responsible for generating clockwise flagellar rotation in Escherichia coli. Molecular Microbiology, 95(2):231-244
Zarbiv G., Li H., Wolf A., Cecchini G., Caplan S. R., Sourjik V. & Eisenbach M. (2012) Energy Complexes Are Apparently Associated with the Switch-Motor Complex of Bacterial Flagella. Journal of Molecular Biology, 416(2):192-207
Liarzi O., Barak R., Bronner V., Dines M., Sagi Y., Shainskaya A. & Eisenbach M. (2010) Acetylation represses the binding of CheY to its target proteins. Molecular Microbiology, 76(4):932-943
Yan J., Barak R., Liarzi O., Shainskaya A. & Eisenbach M. (2008) In vivo acetylation of CheY, a response regulator in chemotaxis of Escherichia coli. Journal of Molecular Biology, 376(5):1260-1271
Cohen-Ben-Lulu G. N., Francis N. R., Shimoni E., Noy D., Davidov Y., Prasad K., Sagi Y., Cecchini G., Johnstone R. M. & Eisenbach M. (2008) The bacterial flagellar switch complex is getting more complex. EMBO Journal, 27(7):1134-1144
Barak R., Yan J., Shainskaya A. & Eisenbach M. (2006) The chemotaxis response regulator CheY can catalyze its own acetylation. Journal of Molecular Biology, 359(2):251-265
Barak R., Prasad K., Shainskaya A., Wolfe A. & Eisenbach M. (2004) Acetylation of the chemicals response regulator CheY by acetyl-CoA synthetase purified from Escherichia coli. Journal of Molecular Biology, 342(2):383-401
Barak R. & Eisenbach M. (2004) Co-regulation of acetylation and phosphorylation of CheY, a response regulator in chemotaxis of Escherichia coli. Journal of Molecular Biology, 342(2):375-381
Sagi Y., Khan S. & Eisenbach M. (2003) Binding of the chemotaxis response regulator CheY to the isolated, intact switch complex of the bacterial flagellar motor - Lack of cooperativity. Journal of Biological Chemistry, 278(28):25867-25871
Barak R. & Eisenbach M. (2001) Acetylation of the response regulator, CheY, is involved in bacterial chemotaxis. Molecular Microbiology, 40(3):731-743
Bren A. & Eisenbach M. (2001) Changing the direction of flagellar rotation in bacteria by modulating the ratio between the rotational states of the switch protein FliM. Journal of Molecular Biology, 312(4):699-709
Barak R. & Eisenbach M. (1999) Chemotactic-like response of Escherichia coli cells lacking the known chemotaxis machinery but containing overexpressed CheY. Molecular Microbiology, 31(4):1125-1137
McEvoy M., Bren A., Eisenbach M. & Dahlquist F. (1999) Identification of the binding interfaces on CheY for two of its targets, the phosphatase CheZ and the flagellar switch protein FliM. Journal of Molecular Biology, 289(5):1423-1433
Bren A. & Eisenbach M. (1998) The N terminus of the flagellar switch protein, FliM, is the binding domain for the chemotactic response regulator, CheY. Journal of Molecular Biology, 278(3):507-514
Prasad K., Caplan S. & Eisenbach M. (1998) Fumarate modulates bacterial flagellar rotation by lowering the free energy difference between the clockwise and counterclockwise states of the motor. Journal of Molecular Biology, 280(5):821-828
Montrone M., Eisenbach M., Oesterhelt D. & Marwan W. (1998) Regulation of switching frequency and bias of the bacterial flagellar motor by CheY and fumarate. Journal of Bacteriology, 180(13):3375-3380
Barak R., Abouhamad W. & Eisenbach M. (1998) Both acetate kinase and acetyl coenzyme A synthetase are involved in acetate-stimulated change in the direction of flagellar rotation in Escherichia coli. Journal of Bacteriology, 180(4):985-988
Blat Y., Gillespie B., Bren A., Dahlquist F. & Eisenbach M. (1998) Regulation of phosphatase activity in bacterial chemotaxis. Journal of Molecular Biology, 284(4):1191-1199
Blat Y. & Eisenbach M. (1996) Conserved C-terminus of the phosphatase CheZ is a binding domain for the chemotactic response regulator CheY. Biochemistry, 35(18):5679-5683
Blat Y. & Eisenbach M. (1996) Mutants with Defective Phosphatase Activity Show No Phosphorylation-dependent Oligomerization of CheZ THE PHOSPHATASE OF BACTERIAL CHEMOTAXIS. Journal of Biological Chemistry, 271(2):1232-1236
Blat Y. & Eisenbach M. (1996) Oligomerization of the Phosphatase CheZ Upon Interaction with the Phosphorylated Form of CheY: THE SIGNAL PROTEIN OF BACTERIAL CHEMOTAXIS. Journal of Biological Chemistry, 271(2):1226-1231
Bren A., Welch M., Blat Y. & Eisenbach M. (1996) Signal termination in bacterial chemotaxis: CheZ mediates dephosphorylation of free rather than switch-bound CheY. Proceedings of the National Academy of Sciences of the United States of America, 93(19):10090-10093
Barak R., Giebel I. & Eisenbach M. (1996) The specificity of fumarate as a switching factor of the bacterial flagellar motor. Molecular Microbiology, 19(1):139-144
Blat Y. & Eisenbach M. (1995) Tar-Dependent and Tar-Independent Pattern-Formation by Salmonella-Typhimurium. Journal of Bacteriology, 177(7):1683-1691
Kumari S., Tishel R., Eisenbach M. & Wolfe A. (1995) Cloning, Characterization, and Functional Expression of Acs, the Gene Which Encodes Acetyl-Coenzyme-a Synthetase in Escherichia-Coli. Journal of Bacteriology, 177(10):2878-2886
Welch M., Margolin Y., Caplan S. & Eisenbach M. (1995) Rotational Asymmetry of Escherichia-Coli Flagellar Motor in the Presence of Arsenate. Biochimica Et Biophysica Acta-Molecular Cell Research, 1268(1):81-87
Karaivanov M., Eisenbach M. & Caplan S. (1995) Fluctuations in Rotation Rate of the Flagellar Motor of Escherichia-Coli. Biophysical Journal, 69(1):250-263
Blat Y. & Eisenbach M. (1994) Phosphorylation-Dependent Binding of the Chemotaxis Signal Molecule CheY to Its Phosphatase, Chez. Biochemistry, 33(4):902-906
Margolin Y., Barak R. & Eisenbach M. (1994) Arsenate Arrests Flagellar Rotation in Cytoplasm-Free Envelopes of Bacteria. Journal of Bacteriology, 176(17):5547-5549
Welch M., Oosawa K., Aizawa S. & Eisenbach M. (1994) Effects of Phosphorylation, Mg2+, and Conformation of the Chemotaxis Protein CheY on Its Binding to the Flagellar Switch Protein Flim. Biochemistry, 33(34):10470-10476
Welch M., Oosawa K., Aizawa S. & Eisenbach M. (1993) Phosphorylation-Dependent Binding of a Signal Molecule to the Flagellar Switch of Bacteria. Proceedings of the National Academy of Sciences of the United States of America, 90(19):8787-8791
Barak R., Welch M., Yanovsky A., Oosawa K. & Eisenbach M. (1992) Acetyladenylate or Its Derivative Acetylates the Chemotaxis Protein CheY Invitro and Increases Its Activity at the Flagellar Switch. Biochemistry, 31(41):10099-10107
Barak R. & Eisenbach M. (1992) Correlation Between Phosphorylation of the Chemotaxis Protein-CheY and Its Activity at the Flagellar Motor. Biochemistry, 31(6):1821-1826
Barak R. & Eisenbach M. (1992) Fumarate or a Fumarate Metabolite Restores Switching Ability to Rotating Flagella of Bacterial Envelopes. Journal of Bacteriology, 174(2):643-645
Rosenbluh A. & Eisenbach M. (1992) Effect of Mechanical Removal of Pili on Gliding Motility of Myxococcus-Xanthus. Journal of Bacteriology, 174(16):5406-5413
Eisenbach M., Constantinou C., Aloni H. & Shinitzky M. (1990) Repellents For Escherichia-Coli Operate Neither by Changing Membrane Fluidity Nor by Being Sensed by Periplasmic Receptors During Chemotaxis. Journal of Bacteriology, 172(9):5218-5224
Eisenbach M., Wolf A., Welch M., Caplan S., Lapidus I., Macnab R., Aloni H. & Asher O. (1990) Pausing, Switching and Speed Fluctuation of the Bacterial Flagellar Motor and Their Relation to Motility and Chemotaxis. Journal of Molecular Biology, 211(3):551-563
Lapidus I., Welch M. & Eisenbach M. (1988) Pausing of Flagellar Rotation is a Component of Bacterial Motility and Chemotaxis. Journal of Bacteriology, 170(8):3627-3632
Ravid S., Matsumura P. & Eisenbach M. (1986) Restoration of Flagellar Clockwise Rotation in Bacterial Envelopes by Insertion of the Chemotaxis Protein CheY. Proceedings of the National Academy of Sciences of the United States of America, 83(19):7157-7161
Eisenbach M., Margolin Y., Ciobotariu A. & Rottenberg H. (1984) Distinction Between Changes in Membrane-Potential and Surface-Charge Upon Chemotactic Stimulation of Escherichia-Coli. Biophysical Journal, 45(2):463-467
Ravid S. & Eisenbach M. (1984) Direction of Flagellar Rotation in Bacterial-Cell Envelopes. Journal of Bacteriology, 158(1):222-230
Ravid S. & Eisenbach M. (1984) Minimal Requirements For Rotation of Bacterial Flagella. Journal of Bacteriology, 158(3):1208-1210
Margolin Y. & Eisenbach M. (1984) Voltage Clamp Effects on Bacterial Chemotaxis. Journal of Bacteriology, 159(2):605-610
Lelkes P., Klein L., Marikovsky Y. & Eisenbach M. (1984) Liposome-Mediated Transfer of Macromolecules Into Flagellated Cell Envelopes from Bacteria. Biochemistry, 23(3):563-568
Eisenbach M. (1983) Changes in membrane potential of Escherichia coli stimulated by galactose. 349-352
Eisenbach M., Raz T. & Ciobotariu A. (1983) A Process Related to Membrane-Potential Involved in Bacterial Chemotaxis to Galactose. Biochemistry, 22(13):3293-3298
Eisenbach M., Zimmerman J., Ciobotariu A., Fischler H. & Korenstein R. (1983) Electric-Field Effects on Bacterial Motility and Chemotaxis. Bioelectrochemistry, 10(6-May):499-510
Ravid S. & Eisenbach M. (1983) Correlation Between Bacteriophage-Chi Adsorption and Mode of Flagellar Rotation of Escherichia-Coli Chemotaxis Mutants. Journal of Bacteriology, 154(2):604-611
Eisenbach M. (1982) Changes in membrane potential of Escherichia coli in response to temporal gradients of chemicals. Biochemistry, 21(26):6818-6825
Eisenbach M. & Adler J. (1981) Bacterial Cell Envelopes with Functional Flagella. Journal of Biological Chemistry, 256(16):8807-8814