Bacterial Chemotoxis

  1. Singh P. K., Sharma P., Afanzar O., Goldfarb M. H., Maklashina E., Eisenbach M., Cecchini G. & Iverson T. M. (2024) CryoEM structures reveal how the bacterial flagellum rotates and switches direction. Nature Microbiology, 9(5):1271-1281
  2. Afanzar O., Di Paolo D., Eisenstein M., Levi K., Plochowietz A., Kapanidis A. N., Berry R. M. & Eisenbach M. (2021) The switching mechanism of the bacterial rotary motor combines tight regulation with inherent flexibility. EMBO Journal, 40(6):104683
  3. Starbird C. A., Tomasiak T. M., Singh P. K., Yankovskaya V., Maklashina E., Eisenbach M., Cecchini G. & Iverson T. M. (2018) New crystal forms of the integral membrane Escherichia coli quinol: fumarate reductase suggest that ligands control domain movement. Journal of Structural Biology, 202(1):100-104
  4. Baron S., Afanzar O. & Eisenbach M. (2017) Methylation-independent adaptation in chemotaxis of Escherichia coli involves acetylation-dependent speed adaptation. FEBS Letters, 591(2):331-337
  5. Maklashina E., Rajagukguk S., Starbird C. A., Mcdonald W. H., Koganitsky A., Eisenbach M., Iverson T. M. & Cecchini G. (2016) Binding of the covalent flavin assembly factor to the flavoprotein subunit of complex II. Journal of Biological Chemistry, 291(6):2904-2916
  6. Fraiberg M., Afanzar O., Cassidy C. K., Gabashvili A., Schulten K., Levin Y. & Eisenbach M. (2015) CheY's acetylation sites responsible for generating clockwise flagellar rotation in Escherichia coli. Molecular Microbiology, 95(2):231-244
  7. Zarbiv G., Li H., Wolf A., Cecchini G., Caplan S. R., Sourjik V. & Eisenbach M. (2012) Energy complexes are apparently associated with the switch-motor complex of bacterial flagella. Journal of Molecular Biology, 416(2):192-207
  8. Liarzi O., Barak R., Bronner V., Dines M., Sagi Y., Shainskaya A. & Eisenbach M. (2010) Acetylation represses the binding of CheY to its target proteins. Molecular Microbiology, 76(4):932-943
  9. Cohen-Ben-Lulu G. N., Francis N. R., Shimoni E., Noy D., Davidov Y., Prasad K., Sagi Y., Cecchini G., Johnstone R. M. & Eisenbach M. (2008) The bacterial flagellar switch complex is getting more complex. EMBO Journal, 27(7):1134-1144
  10. Barak R., Prasad K., Shainskaya A., Wolfe A. & Eisenbach M. (2004) Acetylation of the chemotaxis response regulator CheY by acetyl-CoA synthetase purified from Escherichia coli. Journal of Molecular Biology, 342(2):383-401
  11. Barak R. & Eisenbach M. (1999) Chemotactic-like response of Escherichia coli cells lacking the known chemotaxis machinery but containing overexpressed CheY. Molecular Microbiology, 31(4):1125-1137
  12. McEvoy M. M., Bren A., Eisenbach M. & Dahlquist F. W. (1999) Identification of the binding interfaces on CheY for two of its targets, the phosphatase CheZ and the flagellar switch protein FliM. Journal of Molecular Biology, 289(5):1423-1433
  13. Barak R., Abouhamad W. & Eisenbach M. (1998) Both acetate kinase and acetyl coenzyme A synthetase are involved in acetate-stimulated change in the direction of flagellar rotation in Escherichia coli. Journal of Bacteriology, 180(4):985-988
  14. Prasad K., Caplan S. & Eisenbach M. (1998) Fumarate modulates bacterial flagellar rotation by lowering the free energy difference between the clockwise and counterclockwise states of the motor. Journal of Molecular Biology, 280(5):821-828
  15. Blat Y. & Eisenbach M. (1996) Mutants with Defective Phosphatase Activity Show No Phosphorylation-dependent Oligomerization of CheZ THE PHOSPHATASE OF BACTERIAL CHEMOTAXIS: The phosphatase of bacterial chemotaxis. Journal of Biological Chemistry, 271(2):1232-1236
  16. Blat Y. & Eisenbach M. (1996) Conserved C-terminus of the phosphatase CheZ is a binding domain for the chemotactic response regulator CheY. Biochemistry, 35(18):5679-5683
  17. Kumari S., Tishel R., Eisenbach M. & Wolfe A. J. (1995) Cloning, characterization, and functional expression of acs, the gene which encodes acetyl coenzyme A synthetase in Escherichia coli. Journal of Bacteriology, 177(10):2878-2886
  18. Welch M., Oosawa K., Aizawa S. & Eisenbach M. (1994) Effects of Phosphorylation, Mg2+, and Conformation of the Chemotaxis Protein CheY on Its Binding to the Flagellar Switch Protein Flim. Biochemistry, 33(34):10470-10476
  19. Blat Y. & Eisenbach M. (1994) Phosphorylation-Dependent Binding of the Chemotaxis Signal Molecule CheY to Its Phosphatase, Chez. Biochemistry, 33(4):902-906
  20. Welch M., Oosawa K., Aizawa S. & Eisenbach M. (1993) Phosphorylation-dependent binding of a signal molecule to the flagellar switch of bacteria. Proceedings of the National Academy of Sciences of the United States of America, 90(19):8787-8791
  21. Barak R., Welch M., Yanovsky A., Oosawa K. & Eisenbach M. (1992) Acetyladenylate or Its Derivative Acetylates the Chemotaxis Protein CheY Invitro and Increases Its Activity at the Flagellar Switch. Biochemistry, 31(41):10099-10107
  22. Barak R. & Eisenbach M. (1992) Correlation Between Phosphorylation of the Chemotaxis Protein-CheY and Its Activity at the Flagellar Motor. Biochemistry, 31(6):1821-1826
  23. Eisenbach M., Wolf A., Welch M., Caplan S., Lapidus I., Macnab R., Aloni H. & Asher O. (1990) Pausing, Switching and Speed Fluctuation of the Bacterial Flagellar Motor and Their Relation to Motility and Chemotaxis. Journal of Molecular Biology, 211(3):551-563
  24. Eisenbach M., Constantinou C., Aloni H. & Shinitzky M. (1990) Repellents for Escherichia coli operate neither by changing membrane fluidity nor by being sensed by periplasmic receptors during chemotaxis. Journal of Bacteriology, 172(9):5218-5224
  25. Eisenbach M., Margolin Y., Ciobotariu A. & Rottenberg H. (1984) Distinction Between Changes in Membrane-Potential and Surface-Charge Upon Chemotactic Stimulation of Escherichia-Coli. Biophysical Journal, 45(2):463-467
  26. Ravid S. & Eisenbach M. (1984) Direction of flagellar rotation in bacterial cell envelopes. Journal of Bacteriology, 158(1):222-230
  27. Ravid S. & Eisenbach M. (1984) Minimal requirements for rotation of bacterial flagella. Journal of Bacteriology, 158(3):1208-1210
  28. Margolin Y. & Eisenbach M. (1984) Voltage clamp effects on bacterial chemotaxis. Journal of Bacteriology, 159(2):605-610
  29. Lelkes P., Klein L., Marikovsky Y. & Eisenbach M. (1984) Liposome-Mediated Transfer of Macromolecules Into Flagellated Cell Envelopes from Bacteria. Biochemistry, 23(3):563-568
  30. Eisenbach M., Raz T. & Ciobotariu A. (1983) A Process Related to Membrane-Potential Involved in Bacterial Chemotaxis to Galactose. Biochemistry, 22(13):3293-3298
  31. Ravid S. & Eisenbach M. (1983) Correlation between bacteriophage chi adsorption and mode of flagellar rotation of Escherichia coli chemotaxis mutants. Journal of Bacteriology, 154(2):604-611
  32. Eisenbach M. (1983) Changes in membrane potential of Escherichia coli stimulated by galactose. 349-352
  33. Eisenbach M. (1982) Changes in membrane potential of Escherichia coli in response to temporal gradients of chemicals. Biochemistry, 21(26):6818-6825
  34. Eisenbach M. & Gutman M. (1974) Mid-potential measurements of an unidentified component, controlling the rate of reduction of the b-type cytochromes. FEBS Letters, 46(1-2):368-371