Lei Lei; Kesava Phaneendra Cherukuri; Uria Alcolombri; Diana Meltzer; Dan S. Tawfik (2018). The Dimethylsulfoniopropionate (DMSP) Lyase and Lyase-Like Cupin Family Consists of Bona Fide DMSP lyases as Well as Other Enzymes with Unknown Function.  Biochemistry. 57:(24)3364-3377.  Abstract
Renee Otten; Lin Liu; Lillian R. Kenner; Michael W. Clarkson; David Mavor; Dan S. Tawfik; Dorothee Kern; James S. Fraser (2018). Rescue of conformational dynamics in enzyme catalysis by directed evolution.  Nature Communications. 9.  Abstract


Moshe Goldsmith; Dan S. Tawfik (2017). Enzyme engineering: reaching the maximal catalytic efficiency peak.  Current Opinion in Structural Biology. 47:140-150.  Abstract
Garcia, Lianet Noda Garcia; Maria Luisa Romero Romero; Liam M. Longo; Ilana Kolodkin-Gal; Dan Tawfik (2017). Bacilli glutamate dehydrogenases diverged via coevolution of transcription and enzyme regulation.  EMBO Reports. 18:(7)1139-1149.  Abstract
Dragana Despotovic; Alexander Brandis; Alon Savidor; Yishai Levin; Laura Fumagalli; Dan Tawfik (2017). Diadenosine tetraphosphate (Ap4A) - an E-coli alarmone or a damage metabolite?.  FEBS Journal. 284:(14)2194-2215.  Abstract
Jinrui Gan; Gili Ben-Nissan; Galina Arkind; Mark Tarnavsky; Devin Trudeau; Lianet Noda Garcia; Dan Tawfik; Michal Sharon (2017). Native Mass Spectrometry of Recombinant Proteins from Crude Cell Lysates.  Analytical Chemistry. 89:(8)4398-4404.  Abstract
Moshe Goldsmith; Nidhi Aggarwal; Yacov Ashani et al. (2017). Overcoming an optimization plateau in the directed evolution of highly efficient nerve agent bioscavengers.  Protein Engineering Design & Selection. 30:(4)333-345.  Abstract [All authors]
Uria Alcolombri; Lei Lei; Diana Meltzer; Assaf Vardi; Dan Tawfik (2017). Assigning the Algal Source of Dimethylsulfide Using a Selective Lyase Inhibitor.  ACS Chemical Biology. 12:(1)41-46.  Abstract
Paola Laurino; Dan S. Tawfik (2017). Spontaneous Emergence of S-Adenosylmethionine and the Evolution of Methylation.  Angewandte Chemie-International Edition. 56:(1)343-345.  Abstract


M. Luisa Romero Romero; Avigayel Rabin; Dan Tawfik (2016). Functional Proteins from Short Peptides: Dayhoff's Hypothesis Turns 50.  Angewandte Chemie-International Edition. 55:(52)15966-15971.
Moshe Goldsmith; Yacov Ashani; Raanan Margalit; Abraham Nyska; David Mirelman; Dan S. Tawfik (2016). A new post-intoxication treatment of paraoxon and parathion poisonings using an evolved PON1 variant and recombinant GOT1.  Chemico-Biological Interactions. 259:242-251.  Abstract
Moshe Goldsmith; Simone Eckstein; Yacov Ashani et al. (2016). Catalytic efficiencies of directly evolved phosphotriesterase variants with structurally different organophosphorus compounds in vitro.  Archiv fur Toxikologie. 90:(11)2711-2724.  Abstract [All authors]
Vanja Stojkovic; Lianet Noda-Garcia; Dan S. Tawfik; Danica Galonic Fujimori (2016). Antibiotic resistance evolved via inactivation of a ribosomal RNA methylating enzyme.  Nucleic Acids Research. 44:(18)8897-8907.  Abstract
Devin L. Trudeau; Miriam Kaltenbach; Dan Tawfik (2016). On the Potential Origins of the High Stability of Reconstructed Ancestral Proteins.  Molecular Biology and Evolution. 33:(10)2633-2641.  Abstract
Timo Wille; Katharina Neumaier; Marianne Koller et al. (2016). Single treatment of VX poisoned guinea pigs with the phosphotriesterase mutant C23AL: Intraosseous versus intravenous injection.  Toxicology Letters. 258:198-206.  Abstract [All authors]
Dan Tawfik; Raghavan Varadarajan (2016). Editorial overview: Engineering and design.  Current Opinion in Structural Biology. 39:V-VI.
Daniel N. A. Bolon; David Baker; Dan Tawfik (2016). Special Issue: Special Issue on Protein Evolution and Design July 2016.  Protein Science. 25:(7)1164-1167.
Jeffrey I. Boucher; Daniel N. A. Bolon; Dan Tawfik (2016). Quantifying and understanding the fitness effects of protein mutations: Laboratory versus nature.  Protein Science. 25:(7)1219-1226.  Abstract
Adi Goldenzweig; Moshe Goldsmith; Shannon E. Hill et al. (2016). Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability.  Molecular Cell. 63:(2)337-346.  Abstract [All authors]
Tali Lavy; Hayato Yanagida; Dan Tawfik (2016). Gal3 Binds Gal80 Tighter than Gal1 Indicating Adaptive Protein Changes Following Duplication.  Molecular Biology and Evolution. 33:(2)472-477.  Abstract
Robert G Smock; Itamar Yadid; Orly Dym; Jane Clarke; Dan Tawfik (2016). De Novo Evolutionary Emergence of a Symmetrical Protein Is Shaped by Folding Constraints.  Cell. 164:(3)476-486.  Abstract
Paola Laurino; Liat Rockah-Shmuel; Dan Tawfik (2016). Engineering and Directed Evolution of DNA Methyltransferases.  DNA Methyltransferases - Role and Function. 945:491-509.


Hagit Bar-Rogovsky; Adi Stern; Osnat Penn; Iris Kobl; Tal Pupko; Dan Tawfik (2015). Assessing the prediction fidelity of ancestral reconstruction by a library approach.  Protein Engineering Design & Selection. 28:(11)507-518.  Abstract
Arren Bar-Even; Ron Milo; Elad Noor; Dan Tawfik (2015). The Moderately Efficient Enzyme: Futile Encounters and Enzyme Floppiness.  Biochemistry. 54:(32)
Hayato Yanagida; Ariel Gispan; Noam Kadouri; Shelly Rozen; Michal Sharon; Naama Barkai; Dan S. Tawfik (2015). The Evolutionary Potential of Phenotypic Mutations.  PLoS Genetics. 11:(8)  Abstract
Uria Alcolombri; Shifra Ben-Dor; Ester Feldmesser; Yishai Levin; Dan Tawfik; Assaf Vardi (2015). Identification of the algal dimethyl sulfide-releasing enzyme: A missing link in the marine sulfur cycle.  Science. 348:(6242)1466-1469.  Abstract
Eynat Dellus-Gur; Mikael Elias; Emilia Caselli; Fabio Prati; Merijn L M Salverda; Visser, J Arjan G M De Visser; James S Fraser; Dan Tawfik (2015). Negative Epistasis and Evolvability in TEM-1 beta-Lactamase-The Thin Line between an Enzyme's Conformational Freedom and Disorder.  Journal of Molecular Biology. 427:(14)2396-409.  Abstract
David, Moshe Ben David; Joel Sussman; Christopher L. Maxwel; Klaudia Szeler; Shina C. L. Kamerlin; Dan Tawfik (2015). Catalytic Stimulation by Restrained Active-Site Floppiness-The Case of High Density Lipoprotein-Bound Serum Paraoxonase-1.  Journal of Molecular Biology. 427:(6)1359-1374.  Abstract


Shira Warszawski; Ravit Netzer; Dan Tawfik; Sarel J. Fleishman (2014). A "Fuzzy"-Logic Language for Encoding Multiple Physical Traits in Biomolecules.  Journal of Molecular Biology. 426:(24)4125-4138.  Abstract
Franz Worek; Thomas Seeger; Georg Reiter et al. (2014). Post-exposure treatment of VX poisoned guinea pigs with the engineered phosphotriesterase mutant C23: A proof-of-concept study.  Toxicology Letters. 231:(1)45-54.  Abstract [All authors]
Uria Acolombri; Paola Laurino; Astiaso, Pedro Lara Astiaso; Assaf Vardi; Dan Tawfik (2014). DddD Is a CoA-Transferase/Lyase Producing Dimethyl Sulfide in the Marine Environment.  Biochemistry. 53:(34)5473-5475.  Abstract
Dan Tawfik (2014). Accuracy-rate tradeoffs: how do enzymes meet demands of selectivity and catalytic efficiency?.  Current Opinion in Chemical Biology. 21:73-80.  Abstract
Franz Worek; Thomas Seeger; Moshe Goldsmith; Yacov Ashani; Haim Leader; Joel S. Sussman; Dan Tawfik; Horst Thiermann; Timo Wille (2014). Efficacy of the rePON1 mutant IIG1 to prevent cyclosarin toxicity in vivo and to detoxify structurally different nerve agents in vitro.  Archiv fur Toxikologie. 88:(6)1257-1266.  Abstract
Agnes Toth-Petroczy; Dan Tawfik (2014). The robustness and innovability of protein folds.  Current Opinion in Structural Biology. 26:131-138.  Abstract
Agnes Toth-Petroczy; Dan Tawfik (2014). Hopeful (Protein InDel) Monsters?.  Structure. 22:(6)803-804.
Uria Alcolombri; Mikael Elias; Assaf Vardi; Dan Tawfik (2014). Ambiguous evidence for assigning DddQ as a dimethylsulfoniopropionate lyase and oceanic dimethylsulfide producer.  Proceedings of the National Academy of Sciences of the United States of America. 111:(20)E2078-E2079.
Dan Tawfik (2014). Protein evolution - a reconstructive approach.  FASEB Journal. 28:(1)  Abstract
Mikael Elias; Grzegorz Wieczorek; Shaked Rosenne; Dan Tawfik (2014). The universality of enzymatic rate-temperature dependency.  Trends in Biochemical Sciences. 39:(1)1-7.  Abstract
Liat Rockah-Shmuel; Dan Tawfik; Moshe Goldsmith (2014). Generating Targeted Libraries by the Combinatorial Incorporation of Synthetic Oligonucleotides During Gene Shuffling (ISOR).  Directed Evolution Library Creation: Methods and Protocols, 2nd Edition. 129-137.  Abstract


Izhack Cherny; Per Jr. Greisen; Yacov Ashani; Sagar D. Khare; Gustav Oberdorfer; Haim Leader; David Baker; Dan Tawfik (2013). Engineering V-Type Nerve Agents Detoxifying Enzymes Using Computationally Focused Libraries.  ACS Chemical Biology. 8:(11)2394-2403.  Abstract
Liat Rockah-Shmuel; Agnes Toth-Petroczy; Asaf Sela; Omri Wurtzel; Rotem Sorek; Dan Tawfik (2013). Correlated Occurrence and Bypass of Frame-Shifting Insertion-Deletions (InDels) to Give Functional Proteins.  PLoS Genetics. 9:(10)  Abstract
Hagit Bar-Rogovsky; Adrian Hugenmatter; Dan Tawfik (2013). The Evolutionary Origins of Detoxifying Enzymes THE MAMMALIAN SERUM PARAOXONASES (PONs) RELATE TO BACTERIAL HOMOSERINE LACTONASES.  Journal of Biological Chemistry. 288:(33)23914-23927.  Abstract
Alon Wellner; Maria Raitses Gurevich; Dan Tawfik (2013). Mechanisms of Protein Sequence Divergence and Incompatibility.  PLoS Genetics. 9:(7)  Abstract
Eynat Dellus-Gur; Agnes Toth-Petroczy; Mikael Elias; Dan Tawfik (2013). What Makes a Protein Fold Amenable to Functional Innovation? Fold Polarity and Stability Trade-offs.  Journal of Molecular Biology. 425:(14)2609-2621.  Abstract
Paola Laurino; Dan Tawfik (2013). Engineering DNA methyltransferases for a novel cofactor.  FEBS Journal. 280:173-174.  Abstract
Moshe Goldsmith; Y. Ashani; T. K. Tyagi; David, Moshe Ben David; Haim Leader; Israel Silman; Joel Sussman; Dan Tawfik (2013). Evolved paraoxonases against nerve agents.  FEBS Journal. 280:166-166.  Abstract
Y. Ashani; Moshe Goldsmith; Haim Leader; Grzegorz Wieczorek; Israel Silman; Joel Sussman; Dan Tawfik (2013). The 'thio effect'- a kinetic barrier in OPH-catalyzed hydrolysis of P-S-R containing OPs.  FEBS Journal. 280:167-167.  Abstract
Arren Bar-Even; Dan Tawfik (2013). Engineering specialized metabolic pathways-is there a room for enzyme improvements?.  Current Opinion in Biotechnology. 24:(2)310-319.  Abstract
Agnes Toth-Petroczy; Dan Tawfik (2013). Protein Insertions and Deletions Enabled by Neutral Roaming in Sequence Space.  Molecular Biology and Evolution. 30:(4)761-771.  Abstract
David, Moshe Ben David; Grzegorz Wieczorek; Mikael Elias; Israel Silman; Joel Sussman; Dan Tawfik (2013). Catalytic Metal Ion Rearrangements Underline Promiscuity and Evolvability of a Metalloenzyme.  Journal of Molecular Biology. 425:(6)1028-1038.  Abstract
Moshe Goldsmith; Dan Tawfik (2013). Enzyme Engineering by Targeted Libraries.  Methods In Protein Design. 257-283.  Abstract


Gil Hornung; Raz Bar-Ziv; Dalia Rosin; Nobuhiko Tokuriki; Dan Tawfik; Moshe Oren; Naama Barkai (2012). Noise-mean relationship in mutated promoters.  Genome Research. 22:(12)2409-2417.  Abstract
Liat Rockah-Shmuel; Dan Tawfik (2012). Evolutionary transitions to new DNA methyltransferases through target site expansion and shrinkage.  Nucleic Acids Research. 40:(22)11627-11637.  Abstract
Nobuhiko Tokuriki; Colin J. Jackson; Livnat Afriat-Jurnou; Kirsten T. Wyganowski; Renmei Tang; Dan Tawfik (2012). Diminishing returns and tradeoffs constrain the laboratory optimization of an enzyme.  Nature Communications. 3.  Abstract
Mikael Elias; Alon Wellner; Korina Goldin-Azulay; Eric Chabriere; Julia A. Vorholt; Tobias J. Erb; Dan Tawfik (2012). The molecular basis of phosphate discrimination in arsenate-rich environments.  Nature. 491:(7422)134-137.  Abstract
Yakov Kipnis; Eynat Dellus-Gur; Dan Tawfik (2012). TRINS: a method for gene modification by randomized tandem repeat insertions.  Protein Engineering Design & Selection. 25:(9)437-444.  Abstract
Agnes Toth-Petroczy; Dan Tawfik (2012). Why do some proteins evolve slowly?.  FEBS Journal. 279:530-531.  Abstract
Livnat Afriat-Jurnou; Colin J. Jackson; Dan Tawfik (2012). Reconstructing a Missing Link in the Evolution of a Recently Diverged Phosphotriesterase by Active-Site Loop Remodeling.  Biochemistry. 51:(31)6047-6055.  Abstract
Moshe Goldsmith; Dan Tawfik (2012). Directed enzyme evolution: beyond the low-hanging fruit.  Current Opinion in Structural Biology. 22:(4)406-412.  Abstract
Dan Tawfik (2012). How do proteins evolve?.  Protein Science. 21:61-62.  Abstract
Olga Khersonsky; Gert Kiss; Daniela Roethlisberger; Orly Dym; Shira Albeck; Kendall N. Houk; David Baker; Dan Tawfik (2012). Bridging the gaps in design methodologies by evolutionary optimization of the stability and proficiency of designed Kemp eliminase KE59.  Proceedings of the National Academy of Sciences of the United States of America. 109:(26)10358-10363.  Abstract
David, Moshe Ben David; Mikael Elias; Jean-Jacques Filippi; Elisabet Dunach; Israel Silman; Joel Sussman; Dan Tawfik (2012). Catalytic Versatility and Backups in Enzyme Active Sites: The Case of Serum Paraoxonase 1.  Journal of Molecular Biology. 418:(4-Mar)181-196.  Abstract
Moshe Goldsmith; Yacov Ashani; Yair Simo; David, Moshe Ben David; Haim Leader; Israel Silman; Joel Sussman; Dan Tawfik (2012). Evolved Stereoselective Hydrolases for Broad-Spectrum G-Type Nerve Agent Detoxification.  Chemistry & Biology. 19:(4)456-466.  Abstract
Sagar D. Khare; Yakov Kipnis; Per Jr Greisen et al. (2012). Computational redesign of a mononuclear zinc metalloenzyme for organophosphate hydrolysis.  Nature Chemical Biology. 8:(3)294-300.  Abstract [All authors]
Mikael Elias; Dan Tawfik (2012). Divergence and Convergence in Enzyme Evolution: Parallel Evolution of Paraoxonases from Quorum-quenching Lactonases.  Journal of Biological Chemistry. 287:(1)11-20.  Abstract


Yacov Ashani; Moshe Goldsmith; Haim Leader; Israel Silman; Joel Sussman; Dan Tawfik (2011). In vitro detoxification of cyclosarin in human blood pre-incubated ex vivo with recombinant serum paraoxonases.  Toxicology Letters. 206:(1)24-28.  Abstract
Jordi Camps; Jorge Joven; Bharti Mackness et al. (2011). Paraoxonase-1 and clopidogrel efficacy.  Nature Medicine. 17:(9)1041-1042. [All authors]
Uria Alcolombri; Mikael Elias; Dan Tawfik (2011). Directed Evolution of Sulfotransferases and Paraoxonases by Ancestral Libraries.  Journal of Molecular Biology. 411:(4)837-853.  Abstract
Agnes Toth-Petroczy; Dan Tawfik (2011). Slow protein evolutionary rates are dictated by surface-core association.  Proceedings of the National Academy of Sciences of the United States of America. 108:(27)11151-11156.  Abstract
Arren Bar-Even; Elad Noor; Yonatan Savir; Wolfram Liebermeister; Dan Davidi; Dan Tawfik; Ron Milo (2011). The Moderately Efficient Enzyme: Evolutionary and Physicochemical Trends Shaping Enzyme Parameters.  Biochemistry. 50:(21)4402-4410.  Abstract
Olga Khersonsky; Sergey Malitsky; Ilana Rogachev; Dan Tawfik (2011). Role of Chemistry versus Substrate Binding in Recruiting Promiscuous Enzyme Functions.  Biochemistry. 50:(13)2683-2690.  Abstract
Olga Khersonsky; Daniela Roethlisberger; Andrew M. Wollacott et al. (2011). Optimization of the In-Silico-Designed Kemp Eliminase KE70 by Computational Design and Directed Evolution.  Journal of Molecular Biology. 407:(3)391-412.  Abstract [All authors]
Olga Khersonsky; Daniela Roethlisberger; Andrew M. Wollacott et al. (2011). Optimization of the in silico designed Kemp eliminase KE70 by computational design and directed evolution: Insights from MD.  Abstracts of Papers of the American Chemical Society. 241.  Abstract [All authors]
Merijn L. M. Salverda; Eynat Dellus; Florien A. Gorter; Alfons J. M. Debets; Der Oost, John Van Der Oost; Rolf F. Hoekstra; Dan Tawfik; Visser, J. Arjan G. M. De Visser (2011). Initial Mutations Direct Alternative Pathways of Protein Evolution.  PLoS Genetics. 7:(3)  Abstract
Dan Tawfik; Ronald E. Viola (2011). Arsenate Replacing Phosphate: Alternative Life Chemistries and Ion Promiscuity.  Biochemistry. 50:(7)1128-1134.  Abstract
Rinkoo D. Gupta; Moshe Goldsmith; Yacov Ashani et al. (2011). Directed evolution of hydrolases for prevention of G-type nerve agent intoxication.  Nature Chemical Biology. 7:(2)120-125.  Abstract [All authors]
Itamar Yadid; Dan Tawfik (2011). Functional beta-propeller lectins by tandem duplications of repetitive units.  Protein Engineering Design & Selection. 24:(2-Jan)185-195.  Abstract


Dan Tawfik (2010). Messy biology and the origins of evolutionary innovations.  Nature Chemical Biology. 6:(10)692-696.
Y. Ashani; R. D. Gupta; Moshe Goldsmith; Israel Silman; Joel Sussman; Dan Tawfik; Haim Leader (2010). Stereo-Specific Synthesis Of Analogs Of Nerve Agents And Their Utilization For Selection And Characterization Of Paraoxonase (Pon1) Catalytic Scavengers.  Chemico-Biological Interactions. 187:(1-3)362-369.  Abstract
Misha Soskine; Dan Tawfik (2010). Mutational effects and the evolution of new protein functions.  Nature Reviews Genetics. 11:(8)572-582.  Abstract
Itamar Yadid; Noam Kirshenbaum; Michal Sharon; Orly Dym; Dan Tawfik (2010). Metamorphic proteins mediate evolutionary transitions of structure.  Proceedings of the National Academy of Sciences of the United States of America. 107:(16)7287-7292.  Abstract
Olga Khersonsky; Daniela Rothlisberger; Orly Dym; Shira Albeck; Colin J. Jackson; David Baker; Dan Tawfik (2010). Evolutionary Optimization of Computationally Designed Enzymes: Kemp Eliminases of the KE07 Series.  Journal of Molecular Biology. 396:(4)1025-1042.  Abstract
Leonid Gaidukov; R., I Viji; Shiri Yacobson; Mira Rosenblat; Michael Aviram; Dan Tawfik (2010). ApoE Induces Serum Paraoxonase PON1 Activity and Stability Similar to ApoA-1.  Biochemistry. 49:(3)532-538.  Abstract
Olga Khersonsky; Dan Tawfik (2010). Enzyme Promiscuity: A Mechanistic and Evolutionary Perspective.  Annual Review Of Biochemistry, Vol 79. 471-505.  Abstract


C. J. Jackson; J. -L. Foo; Nobuhiko Tokuriki; L. Afriat; P. D. Carr; H. -K. Kim; G. Schenk; Dan Tawfik; D. L. Ollis (2009). Conformational sampling, catalysis, and evolution of the bacterial phosphotriesterase.  Proceedings of the National Academy of Sciences of the United States of America. 106:(51)21631-21636.  Abstract
Kalia Bernath Levin; Orly Dym; Shira Albeck; Shlomo Magdassi; Anthony H. Keeble; Colin Kleanthous; Dan Tawfik (2009). Following evolutionary paths to protein-protein interactions with high affinity and selectivity.  Nature Structural & Molecular Biology. 16:(10)1049-U67.  Abstract
Nobuhiko Tokuriki; Dan Tawfik (2009). Stability effects of mutations and protein evolvability.  Current Opinion in Structural Biology. 19:(5)596-604.  Abstract
Keith V. Wood; Dan Tawfik (2009). Protein technologies.  Current Opinion in Biotechnology. 20:(4)381-382.
Dan Tawfik (2009). Protein evolution - a reconstructive approach.  FEBS Journal. 276:15-15.  Abstract
Olga Khersonsky; Mira Rosenblat; Lilly Toker; Shiri Yacobson; Adrian Hugenmatter; Israel Silman; Joel Sussman; Michael Aviram; Dan Tawfik (2009). Directed Evolution of Serum Paraoxonase PON3 by Family Shuffling and Ancestor/Consensus Mutagenesis, and Its Biochemical Characterization.  Biochemistry. 48:(28)6644-6654.  Abstract
Nobuhiko Tokuriki; Dan Tawfik (2009). Chaperonin overexpression promotes genetic variation and enzyme evolution.  Nature. 459:(7247)668-U71.  Abstract
Nobuhiko Tokuriki; Dan Tawfik (2009). Protein Dynamism and Evolvability.  Science. 324:(5924)203-207.  Abstract
Nobuhiko Tokuriki; Christopher J. Oldfield; Vladimir N. Uversky; Igor N. Berezovsky; Dan Tawfik (2009). Do viral proteins possess unique biophysical features?.  Trends in Biochemical Sciences. 34:(2)53-59.  Abstract
Dan Tawfik (2009). Amidation of Carboxyl Groups.  Protein Protocols Handbook, Third Edition. 865-866.
Dan Tawfik (2009). Amidination of Lysine Side Chains.  Protein Protocols Handbook, Third Edition. 867-869.
Dan Tawfik (2009). Ethoxyformylation of Histidine.  Protein Protocols Handbook, Third Edition. 859-861.
Dan Tawfik (2009). Modification of Arginine Side Chains with p-Hydroxyphenylglyoxal.  Protein Protocols Handbook, Third Edition. 863-864.
Dan Tawfik (2009). Modification of Sulfhydryl Groups with DTNB.  Protein Protocols Handbook, Third Edition. 873-874.
Dan Tawfik (2009). Modification of Tryptophan with 2-Hydroxy-5-Nitrobenzylbromide.  Protein Protocols Handbook, Third Edition. 871-872.
Dan Tawfik (2009). Nitration of Tyrosines.  Protein Protocols Handbook, Third Edition. 855-858.
Dan Tawfik (2009). Side Chain Selective Chemical Modifications of Proteins.  Protein Protocols Handbook, Third Edition. 851-854.


Shimon Bershtein; Dan Tawfik (2008). Ohno's Model Revisited: Measuring the Frequency of Potentially Adaptive Mutations under Various Mutational Drifts.  Molecular Biology and Evolution. 25:(11)2311-2318.  Abstract
Rinkoo D. Gupta; Dan Tawfik (2008). Directed enzyme evolution via small and effective neutral drift libraries.  Nature Methods. 5:(11)939-942.  Abstract
Shimon Bershtein; Korina Goldin; Dan Tawfik (2008). Intense neutral drifts yield robust and evolvable consensus proteins.  Journal of Molecular Biology. 379:(5)1029-1044.  Abstract
Daniela Rothlisberger; Olga Khersonsky; Andrew M. Wollacott et al. (2008). Kemp elimination catalysts by computational enzyme design.  Nature. 453:(7192)190-U4.  Abstract [All authors]
Shimon Bershtein; Dan Tawfik (2008). Advances in laboratory evolution of enzymes.  Current Opinion in Chemical Biology. 12:(2)151-158.  Abstract
Leonid A. Gaidukov; Amir Aharoni; Olga Khersonsky; Dan Tawfik (2008). Serum paraoxonase PON1 and its interactions with HDL.  FASEB Journal. 22.  Abstract
Nobuhiko Tokuriki; Francois Stricher; Luis Serrano; Dan Tawfik (2008). How Protein Stability and New Functions Trade Off.  PLoS Computational Biology. 4:(2)  Abstract


Sergio G. Peisajovich; Dan Tawfik (2007). Protein engineers turned evolutionists.  Nature Methods. 4:(12)991-994.  Abstract
Leonid Gaidukov; Dan Tawfik (2007). The development of human sera tests for HDL-bound serum PON1 and its lipolactonase activity.  Journal of Lipid Research. 48:(7)1637-1646.  Abstract
Moshe Goldsmith; Csaba Kiss; Andrew R. M. Bradbury; Dan Tawfik (2007). Avoiding and controlling double transformation artifacts.  Protein Engineering Design & Selection. 20:(7)315-318.  Abstract
Nobuhiko Tokuriki; Francois Stricher; Joost Schymkowitz; Luis Serrano; Dan Tawfik (2007). The stability effects of protein mutations appear to be universally distributed.  Journal of Molecular Biology. 369:(5)1318-1332.  Abstract
Gil Amitai; Rinkoo Devi Gupta; Dan Tawfik (2007). Latent evolutionary potentials under the neutral mutational drift of an enzyme.  Frontiers in Life Science. 1:(1)67-78.  Abstract
Asael Herman; Dan Tawfik (2007). Incorporating Synthetic Oligonucleotides via Gene Reassembiv (ISOR): a versatile tool forgenerating targeted libraries.  Protein Engineering Design & Selection. 20:(5)219-226.  Abstract
Itamar Yadid; Dan Tawfik (2007). Reconstruction of functional beta-propeller lectins via homo-oligomeric assembly of shorter fragments.  Journal of Molecular Biology. 365:(1)10-17.  Abstract


Shimon Bershtein; Michal Segal; Roy Bekerman; Nobuhiko Tokuriki; Dan Tawfik (2006). Robustness-epistasis link shapes the fitness landscape of a randomly drifting protein.  Nature. 444:(7121)929-932.  Abstract
Livnat Afriat; Cintia Roodveldt; Giuseppe Manco; Dan Tawfik (2006). The latent promiscuity of newly identified microbial lactonases is linked to a recently diverged phosphotriesterase.  Biochemistry. 45:(46)13677-13686.  Abstract
Leonid Gaidukov; Mira Rosenblat; Michael Aviram; Dan Tawfik (2006). The 192R/Q polymorphs of serum paraoxonase PON1 differ in HDL binding, lipolactonase stimulation, and cholesterol efflux.  Journal of Lipid Research. 47:(11)2492-2502.  Abstract
Olga Khersonsky; Cintia Roodveldt; Dan Tawfik (2006). Enzyme promiscuity: evolutionary and mechanistic aspects.  Current Opinion in Chemical Biology. 10:(5)498-508.  Abstract
Joel Sussman; Amir Aharoni; Michal Harel et al. (2006). 3D structure of mammalian paraoxonase at 2.2A resolution.  Toxicology Letters. 164:S9-S9.  Abstract [All authors]
Andrew D. Griffiths; Dan Tawfik (2006). Miniaturising the laboratory in emulsion droplets.  Trends in Biotechnology. 24:(9)395-402.  Abstract
R Williams; Sg Peisajovich; Oj Miller; S Magdassi; Dan Tawfik; Ad Griffiths (2006). Amplification of complex gene libraries by emulsion PCR.  Nature Methods. 3:(7)545-550.  Abstract
Oj Miller; K Bernath; Jj Agresti et al. (2006). Directed evolution by in vitro compartmentalization.  Nature Methods. 3:(7)561-570.  Abstract [All authors]
Dan Tawfik (2006). Structure mechanism and directed evolution of serum paraoxonases - a family of detoxifying and antiarteriosclerotic enzymes.  FEBS Journal. 273:15-15.  Abstract
Jonathan Garel; Dan Tawfik (2006). Mechanism of hydrolysis and aminolysis of homocysteine thiolactone.  Chemistry-A European Journal. 12:(15)4144-4152.  Abstract
Gil Amitai; L Gaidukov; R Adani et al. (2006). Enhanced stereoselective hydrolysis of toxic organophosphates by directly evolved variants of mammalian serum paraoxonase.  FEBS Journal. 273:(9)1906-1919.  Abstract [All authors]
Olga Khersonsky; Dan Tawfik (2006). The histidine 115-histidine 134 dyad mediates the lactonase activity of mammalian serum paraoxonases.  Journal of Biological Chemistry. 281:(11)7649-7656.  Abstract
M Rosenblat; L Gaidukov; Olga Khersonsky; J Vaya; Roni Oren; Dan Tawfik; M Aviram (2006). The catalytic histidine dyad of high density lipoprotein-associated serum paraoxonase-1 (PON1) is essential for PON1-mediated inhibition of low density lipoprotein oxidation and stimulation of macrophage cholesterol efflux.  Journal of Biological Chemistry. 281:(11)7657-7665.  Abstract
Sg Peisajovich; L Rockah; Dan Tawfik (2006). Evolution of new protein topologies through multistep gene rearrangements.  Nature Genetics. 38:(2)168-174.
Dan Tawfik (2006). Loop grafting and the origins of enzyme species.  Science. 311:(5760)475-476.
Olga Khersonsky; Dan Tawfik (2006). Chromogenic and fluorogenic assays for the lactonase activity of serum paraoxonases.  ChemBioChem. 7:(1)49-53.


L Gaidukov; Dan Tawfik (2005). High affinity, stability, and lactonase activity of serum paraoxonase PON1 anchored on HDL with ApoA-I.  Biochemistry. 44:(35)11843-11854.  Abstract
C Roodveldt; Dan Tawfik (2005). Shared promiscuous activities and evolutionary features in various members of the amidohydrolase superfamily.  Biochemistry. 44:(38)12728-12736.  Abstract
Lc James; Dan Tawfik (2005). Structure and kinetics of a transient antibody binding intermediate reveal a kinetic discrimination mechanism in antigen recognition.  Proceedings of the National Academy of Sciences of the United States of America. 102:(36)12730-12735.  Abstract
Olga Khersonsky; Dan Tawfik (2005). Structure-reactivity studies of serum paraoxonase PON1 suggest that its native activity is lactonase.  Biochemistry. 44:(16)6371-6382.  Abstract
Sm Gould; Dan Tawfik (2005). Directed evolution of the promiscuous esterase activity of carbonic anhydrase II.  Biochemistry. 44:(14)5444-5452.  Abstract
Dan Tawfik (2005). Protein evolution: The theory and the practice.  Abstracts of Papers of the American Chemical Society. 229:U1175-U1175.  Abstract
K Bernath; S Magdassi; Dan Tawfik (2005). Directed evolution of protein inhibitors of DNA-nucleases by in vitro compartmentalization (IVC) and nano-droplet delivery.  Journal of Molecular Biology. 345:(5)1015-1026.  Abstract
C Roodveldt; Dan Tawfik (2005). Directed evolution of phosphotriesterase from Pseudomonas diminuta for heterologous expression in Escherichia coli results in stabilization of the metal-free state.  Protein Engineering Design & Selection. 18:(1)51-58.  Abstract
Hm Cohen; Ad Griffiths; Dan Tawfik; D Loakes (2005). Determinants of cofactor binding to DNA methyltransferases: insights from a systematic series of structural variants of S-adenosylhomocysteine.  Organic & Biomolecular Chemistry. 3:(1)152-161.  Abstract


Michal Harel; Amikam Aharoni; L Gaidukov et al. (2004). Structure And Evolution Of The Serum Paraoxonase Family Of Detoxifying And Anti-Atherosclerotic Enzymes.  Nature Structural & Molecular Biology. 11:(5)412-419.  Abstract [All authors]
M Hai; K Bernath; Dan Tawfik; S Magdassi (2004). Flow cytometry: A new method to investigate the properties of water-in-oil-in-water emulsions.  Langmuir. 20:(6)2081-2085.  Abstract
K Bernath; Mt Hai; E Mastrobattista; Ad Griffiths; S Magdassi; Dan Tawfik (2004). In vitro compartmentalization by double emulsions: sorting and gene enrichment by fluorescence activated cell sorting.  Analytical Biochemistry. 325:(1)151-157.  Abstract
Amikam Aharoni; L Gaidukov; S Yagur; Lily Toker; Israel Silman; Dan Tawfik (2004). Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization.  Proceedings of the National Academy of Sciences of the United States of America. 101:(2)482-487.  Abstract
Hm Cohen; Dan Tawfik; Ad Griffiths (2004). Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalization.  Protein Engineering Design & Selection. 17:(1)3-11.  Abstract


Lc James; Dan Tawfik (2003). The specificity of cross-reactivity: Promiscuous antibody binding involves specific hydrogen bonds rather than nonspecific hydrophobic stickiness.  Protein Science. 12:(10)2183-2193.  Abstract
Lc James; Dan Tawfik (2003). Conformational diversity and protein evolution - a 60-year-old hypothesis revisited.  Trends in Biochemical Sciences. 28:(7)361-368.  Abstract
Lc James; P Roversi; Dan Tawfik (2003). Antibody multispecificity mediated by conformational diversity.  Science. 299:(5611)1362-1367.  Abstract
Ad Griffiths; Dan Tawfik (2003). Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization.  EMBO Journal. 22:(1)24-35.  Abstract


A Sepp; Dan Tawfik; Ad Griffiths (2002). Microbead display by in vitro compartmentalisation: selection for binding using flow cytometry.  FEBS Letters. 532:(3)455-458.  Abstract
Yf Lee; Dan Tawfik; Ad Griffiths (2002). Investigating the target recognition of DNA cytosine-5 methyltransferase HhaI by library selection using in vitro compartmentalisation.  Nucleic Acids Research. 30:(22)4937-4944.  Abstract
Hm Cohen; Dan Tawfik; Ad Griffiths (2002). Promiscuous methylation of non-canonical DNA sites by HaeIII methyltransferase.  Nucleic Acids Research. 30:(17)3880-3885.  Abstract
Ab Lindner; Sh Kim; Dg Schindler; Zelig Eshhar; Dan Tawfik (2002). Esterolytic antibodies as mechanistic and structural models of hydrolases - A quantitative analysis.  Journal of Molecular Biology. 320:(3)559-572.  Abstract


Lc James; Dan Tawfik (2001). Catalytic and binding poly-reactivities shared by two unrelated proteins: The potential role of promiscuity in enzyme evolution.  Protein Science. 10:(12)2600-2607.  Abstract
F Hollfelder; Aj Kirby; Dan Tawfik (2001). On the magnitude and specificity of medium effects in enzyme-like catalysts for proton transfer.  Journal of Organic Chemistry. 66:(17)5866-5874.  Abstract


Ab Lindner; Zelig Eshhar; Dan Tawfik (1999). Conformational changes affect binding and catalysis by ester-hydrolysing antibodies.  Journal of Molecular Biology. 285:(1)421-430.  Abstract


Sh Kim; Dg Schindler; Ab Lindner; Dan Tawfik; Zelig Eshhar (1997). Expression and characterization of recombinant single-chain Fv and Fv fragments derived from a set of catalytic antibodies.  Molecular Immunology. 34:(13-Dec)891-906.  Abstract
Dan Tawfik; Ab Lindner; R Chap; Zelig Eshhar; Bs Green (1997). Efficient and selective p-nitrophenyl-ester-hydrolyzing antibodies elicited by a p-nitrobenzyl phosphonate hapten.  FEBS Journal. 244:(2)619-626.  Abstract
Jb Charbonnier; B Golinellipimpaneau; B Gigant et al. (1997). Structural convergence in the active sites of a family of catalytic antibodies.  Science. 275:(5303)1140-1142.  Abstract [All authors]


Dan Tawfik; R Rapley (1995). Perspectives in Protein Engineering and Complementary Technologies, Oxford, uk, September 13-17, 1994 - to Design or Select - That is the Question.  Molecular Biotechnology. 3:(2)172-173.
Dan Tawfik; R Chap; Bs Green; M Sela; Zelig Eshhar (1995). Unexpectedly High Occurrence of Catalytic Antibodies in Mrl/Lpr and Sjl Mice Immunized With a Transition-State Analog - is There a Linkage to Autoimmunity.  Proceedings of the National Academy of Sciences of the United States of America. 92:(6)2145-2149.  Abstract


B Golinellipimpaneau; B Gigant; T Bizebard et al. (1994). Crystal-Structure of a Catalytic Antibody Fab With Esterase-Like Activity.  Structure. 2:(3)175-183.  Abstract [All authors]
Dan Tawfik; R Chap; Zelig Eshhar; Bs Green (1994). Ph on-Off Switching of Antibody Hapten Binding by Site-Specific Chemical Modification of Tyrosine.  Protein Engineering. 7:(3)431-434.  Abstract
R Zemel; Dg Schindler; Dan Tawfik; Zelig Eshhar; Bs Green (1994). Differences in the Biochemical-Properties of Esterolytic Antibodies Correlate With Structural Diversity.  Molecular Immunology. 31:(2)127-137.  Abstract


Dan Tawfik; Zelig Eshhar; A Bentolila; Bs Green (1993). 1,8-Diazabicyclo[5.4.0]Undecene Mediated Transesterification of p-Nitrophenyl Phosphonates - a Novel Route to Phosphono Esters.  Synthesis-Stuttgart. (10)968-972.  Abstract
Dan Tawfik; Bs Green; R Chap; M Sela; Zelig Eshhar (1993). Catelisa - a Facile General-Route to Catalytic Antibodies.  Proceedings of the National Academy of Sciences of the United States of America. 90:(2)373-377.  Abstract
Dan Tawfik; Zelig Eshhar; Bs Green (1993). From Phosphonates to Catalytic Antibodies - a Novel Route to Phosphonoester Transition-State Analogs and Haptens.  Phosphorus Sulfur And Silicon And The Related Elements. 76:(4-Jan)383-386.  Abstract


Dan Tawfik; Bs Green; Zelig Eshhar (1992). Detection of Catalytic Monoclonal-Antibodies.  Analytical Biochemistry. 202:(1)35-39.


Dan Tawfik; Rr Zemel; R Aradyellin; Bs Green; Zelig Eshhar (1990). Simple Method For Selecting Catalytic Monoclonal-Antibodies That Exhibit Turnover and Specificity.  Biochemistry. 29:(42)9916-9921.