Voltage imaging of neural computations


Voltage imaging is an emerging technology that enables optical recording of membrane potentials and spiking activities of neurons at millisecond timescale. Light-sheet microscopy in behaving larval zebrafish holds the potential for expanding this technology to a brain-wide scale and elevating the level of understanding of neural computations during animal behaviors.

Based on the emergence of a bright, photostable voltage indicator in 2019,  we have been developing optical, genetic and computational methods for advancing this technology in collaboration with other laboratories.


Related literature:

[1] Abdelfattah*, Kawashima* et al.
    "Bright and photostable chemigenetic indicators for extended in vivo voltage imaging"
    Science. 365 (6454): 699-704 (2019) 

[2] Freeman et al.,
     “Mapping brain activity at scale with cluster computing.”
     Nature Methods. 11(9):941-50 (2014).

[3] Vladimirov et al.,
     “Light-sheet functional imaging in fictively behaving zebrafish.”
     Nature Methods. 11(9):883-4 (2014).