We are studying new modalities of optical microscopy based on interaction of matter with short intense optical pulses. These techniques in general offer optical sectioning and live imaging without the need for labeling or fixing the specimens.
We have developed third-harmonic generation (THG) microscopy, which resolve structural information via spatial changes of the nonlinear susceptibility. We also employed techniques based on coherent control and optical pulse shaping for molecular imaging via coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS).
We developed techniques that use short pulse manipulation for microscopy application. Temporal focusing microscopy uses the controlled compression of the pulse to achieve scanningless nonlinear microscopy. We also study the interaction of short pulses with scattering environment such as biological tissue.