DNA Mutagenesis

DNA mutagenesis is a powerful tool for manipulating target genes. DNA mutagenesis includes single or multiple residue modifications, as well as, insertion and deletion. 
At the ISPC we perform diverse molecular manipulations using RF/TPCR (Erijman et al., Erijman, Shifman and Peleg):

  • Generation of a single targeted mutation.
  • Simultaneous generation of multiple targeted mutations at distinct positions.
  • Generation of targeted and combinatorial libraries.
  • Deletion of sequences from a plasmid.
  • Simultaneous insertion and deletion. 

Schematic presentation of DNA cloning by Transfer-PCR (TPCR).