Capitalizing on the versatility of MRI contrast mechanisms, we design novel synthetic probes for molecular and metabolic imaging, enabling visualization of biological processes in vivo. These synthetic agents employ diverse strategies to generate specific MRI readouts, including modulating water relaxation properties, capturing proton exchange processes, or utilizing non-proton MRI-active nuclei, such as deuterium and fluorine-19. By harnessing MRI’s chemical shift encoding capabilities of the synthetic probes, we can simultaneously monitor multiple events and present them as artificial MRI colors for multiplex imaging purposes.