Optogenetic tool development
Optogenetics uses light-activated proteins to rapidly and reversibly modify the activity of living neurons. We have developed several new optogenetic tools, mostly focusing on light-gated silencing of neural and synaptic activity. We recently developed the mosquito-derived eOPN3 for presynaptic silencing, and PdCO is our new bidirectionally-switchable inhibitory optoGPCR, which allows a wide range of multiplexed applications. For more information on optogenetics, please see our recent reviews (e.g. Wiegert et al., 2017; Rost, Wietek et al., 2022). Our constructs (plasmids and AAV preps) are available on Addgene. Selected constructs are also available as packaged AAVs on the ETH viral vector facility.