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Jacob Hanna Lab
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Data & Protocols

Ex utero Mouse Embryogenesis Nature 2021; Synthetic Embryos Cell 2022

  • Highly Conducive Ex utero Mouse Embryogenesis from Pre-Gastrulation to Late Organogenesis
  • Components to Assemble Electronically Controlled Ex Utero Embryo Culture Platform

HENSM Bayerl et al Cell Stem Cell 2021

  • HENSM media conditions

Gafni & Weinberger et al Nature 2013, Irie & Weinberger et al Cell 2015

  • WIS-NHSM human naive medium formulation & protocols
  • Preparation of human LIF
  • Human naive PSC embryo microinjection and generation of humanized cross species chimeras
  • Recombinant vitronectin production
  • In house and custom production of B27 supplement

Rais et al Nature 2013

  • Optimized mouse secondary fibroblast reprogramming protocol involving Mbd3 inhibition with siRNA
  • Mouse deterministic iPSC reprogramming with secondary OKSM and constitutive hypomorphic expression of Mbd3
  • Human secondary and BJ fibroblast reprogramming protocol with OSKML-Mbd3 siRNA
  • Robust reprogramming of human Naive iPSCs form adult human primary fibroblasts involving Mbd3 depletion

Hanna Lab Addgene Plasmids Library

  • Library @ Addgene
Weizmann Institute of Science Department of Molecular Genetics
Jacob Hanna Lab
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