The Dana and Yossie Hollander Center for Structural Proteomics (ISPC) is a national center, that implements all steps in the pipeline from the gene to the 3D protein structure. The center collaborates with and provides service for the scientific community and for clinicians by making available its expertise in DNA manipulation, protein expression, protein purification and protein structure determination and analysis. In addition, large quantities of purified proteins are produced for biochemical and structural studies. The ISPC is constantly engaged in development of new methodologies, as well as in the implementation of state-of-the-art technologies to deal with challenging scientific questions. In addition, the center provides consultation and guidance to scientists at all stages of the pipeline.