Protein Expression unit
Dr. Tamar Unger, Head
Benoziyo Biochemistry Building, room 117
DNA Manipulation unit
Dr. Yoav Peleg, Head
Benoziyo Biochemistry Building, room 116
Target genes of interest cloned in appropriate expression vectors are introduced into host cells. To increase the probability that a given protein will be expressed in a soluble and active form, and in high yield, several expression systems are used. These include bacterial systems and three eukaryotic systems - yeast, insect and mammalian cells. Expression is screened on a small scale in 4-10 ml cultures, and soluble and insoluble cellular fractions are analyzed by SDS-PAGE for protein expression. Once optimal conditions have been determined, large-scale cultures (1-5L) are used to obtain greater amounts of protein.