DNA Manipulation unit
Dr. Yoav Peleg, Head
Benoziyo Biochemistry Building, room 116
Protein Expression unit
Dr. Tamar Unger, Head
Benoziyo Biochemistry Building, room 117
DNA cloning and sequence manipulation are essential tools in all life science disciplines. The Restriction Free (RF) and Transfer-PCR (TPCR) cloning methodologies are gaining momentum as alternative approaches to the traditional cloning methods, which are based on restriction endonucleases followed by a ligation step. Since their implementation, the applications of the RF/TPCR methodologies have been extended beyond their original utilization for single gene cloning. The strength of these methods lies in their simplicity and flexibility, which can be harnessed for a large spectrum of applications. These include cloning of multiple DNA fragments, insertion and/or deletion of specific DNA sequences, and single and multiple-site mutagenesis.