Breast carcinoma and neutrophils in lung: LCM

Imaging of CMTMR labeled breast carcinoma cells (red) and their surrounding neutrophils by light sheet microscopy of whole lungs

Endogenous neutrophils were labeled 5 mins before harvesting the lungs with Alexa 647-labeled anti-Ly6G mAb. Lungs were inflated with low melting temperature agarose, excised and cleared. Autofluorescent lung stromal cells are depicted in green. Bar= 100µm.