Murine tumor cell TEM across murine ECs

Hoechst labeled breast cancer cell (E0771) in the process of crossing a confluent monolayer of bEnd3 murine endothelial cells.
Hoechst labeled breast cancer cell (E0771) in the process of crossing a confluent monolayer of bEnd3 murine endothelial cells.

Phase contrast and fluorescence microscopy. Note the very slow generation of a sub-endothelial leading edge by the transmigrating tumor cell followed by a slow squeezing of the tumor nucleus through the endothelial junction.