Ultrafast NMR is a single scan technique capable of delivering arbitrary multidimensional spectra, within a fraction of a second. Ultrafast 2D NMR operates on the basis of joint spectroscopic and imaging concepts and manipulations of the spin magnetization. By reducing acquisition times from minutes to sub-seconds ultrafast 2D NMR allows one to monitor a series of hitherto unavailable processes, like the evolution and dynamics of a chemical reaction while it is in progress or "snapshots" of a biophysical rearrangement in real time.
15N-1H 2D HMQC NMR spectrum of 15N-labeled Ubiqiutin recorded at a concentration of 2.3 mM and with an acquistion time of 85 ms.
13C-1H 2D HSQC NMR spectrum of U(15N,13C)-protein A recorded at a concentration of 1.0 mM and with an acquisition time of 60 ms.