Facilities

Our Crystallography Facilities offer state-of-the-art equipment and expert advice and training in the use of crystallogenesis for structural biology and small molecules.

Our Bio- Nuclear Magnetic Resonance (NMR) unit is equipped with high resolution instruments (e.g., 1000 MHz (1 GHz) ultrahigh field NMR spectrometer, UltraShield Bruker AVANCE NEO-600) and offers training and guidance in the use of NMR in structural biology.

Our Electron Microscopy unit houses state-of-the-art electron microscopy instrumentation for single particle cryo-EM, cryo-ET, and cryo-STEM. The unit provides services and training, along with leading experts in the field who can assist with sample preparation, data-collection, and processing. Equipment includes Titan Krios and Talos Arctica microscopes equipped with the latest direct detectors.

Single-Molecule Fluorescence Spectroscopy combined with FRET (Förster resonance energy transfer), is a method that provides information on the structural changes of proteins and DNA at timescales from nanoseconds to seconds. It is an ideal tool to study extremely flexible intrinsically disordered proteins and to probe molecular dynamics in biomolecular condensates and cells. The Hoffmann group expertise in SM-FRET. Advanced fluorescence services and training are also available at our Bio Imaging unit.

High-Speed Atomic Force Microscopy (HS-AFM) is a cutting-edge technique in protein biophysics, allowing us to acquire “videos” of proteins under near native conditions. HS-AFM uniquely provides us both structural and dynamic information of proteins on a single-molecule level. It does so by quickly measuring the topography of a surface allowing for the direct imaging of the proteins in aqueous solutions, ambient temperature, without labelling or staining procedures. The Lanksy group uses HS-AFM to study membrane proteins. Our AFM services facilities are at the nanofabrication unit.

Our state-of-the-art Scienion sciFLEXARRAYER S12 utilizes piezo dispense capillary (PDC) technology to enable dispensing of picolitre liquids onto substrate surfaces with micrometer spatial precision. Full automation allows the rapid and efficient generation of complex microarrays containing hundreds of unique samples. The Afek group routinely use these capabilities for the in-house production of custom DNA arrays for the high-throughput study of protein-DNA interactions.

Our department is equipped with a plethora of techniques to study macromolecules and macromolecular complexes. The booking system for internal users is supplied in this link.

In addition, our Protein Analysis unit offers advanced services and training in biomolecular interactions (e.g., Biacore, ITC, MST) and protein characterization (e.g., DSC, DLS, DSF, FFF).