December 13, 1988 - December 13, 2021
G-INCPM-Special Seminar - Dr. Shalev Itzkovitz, Dept. of Molecular Cell Biology, Weizmann - "Spatial Transcriptomics of Mammalian Tissues"
11:00 - 12:30
Nancy and Stephen Grand Israel National Center for Personalized Medicine
Dr. Shalev Itzkovitz
Dept.of Molecular Cell Biology, Weizmann
|Abstract||Show full text abstract about Mammalian tissues are often composed of repeating anatomical...»|
Mammalian tissues are often composed of repeating anatomical units that are polarised by morphogens or directional blood flow. Thus single cells at different tissue coordinates operate in distinct microenvironment.
This spatial diversity enables optimisation of tissue function by allocating different tasks to cells that reside in distinct tissue locations. To explore this spatial division of labor on a genome-wide scale we are combining single cell transcriptomics with in-situ measurements in intact tissues to enable inference of the sequenced cells’ original tissue locations. This approach enables reconstructing global spatial gene expression atlases. I will demonstrate these approaches on two stereotypical and highly structured organs - the liver and the intestinal epithelium.