Antibodies are a family of vertebrate proteins capable of selective recognition of almost any epitope, making them of great interest in biotechnological and therapeutic applications. However, many antibodies possess poor biophysical properties, leading to misfolding and aggregation, as well as unwanted non-specific interactions. In order increase the ease and speed with which an antibody can be optimised, and to solve issues associated with using single-chain antibody fragments (scFvs), we developed an automated pipeline for the re-engineering of antibody variable fragments directly from sequence.
