LabMaster/PhenoMaster; room 334 (BioHazard)
The LabMaster/PhenoMaster systems (TSE Systems) are a comprehensive laboratory animal monitoring systems that allows simultaneous monitoring of food intake, energy expenditure and locomotor activity. The calorimetric system is an open-circuit system that determines O2 consumption, CO2 production, heat production and respiratory exchange ratio (RER). The instrument consists of a combination of sensitive feeding and drinking sensors for automated online measurement. Four sensors are allocated per cage for preference experiments. A photo beam-based activity monitoring system detects and records ambulatory movements, including rearing and climbing, in every cage. Two systems, each one includes eight cages, are available. One of the systems is equipped with a special food access apparatus that enables the usage of various food restrictions regimes. Mice should be trained for several days in specialized training boxes prior to testing in the metabolic cages. At the end of the experiment, the relevant data is exported and assistance in analyzing the data is offered.
CaloTreadmill; room 334 (BioHazard)
The CaloTreadmill (TSE Systems) is a treadmill with sealed housing for calorimetric measurements during treadmill exercise. When combined with the LabMaster system it is also able to measure O2 and CO2 consumption during forced exercise.
Body composition
The Bruker’s minispec plus NMR based body composition allows body composition analysis (lean mass and fat mass) of conscious mice. Body composition analysis is recommended to all animals that go through the metabolic cages in order to normalize the values to the lean weight.
FeedTime; room 335 (BioHazard)
The FeedTime (TSE Systems) a system that allows controlling the time in which food is accessible for the mice. Our system includes 16 boxes (larger than the regular ones) that allow group/single housing.
Running wheels; room 335 (BioHazard)
Running wheel activity may serve as a way for assessing general locomotion, circadian activity and may used to induce activity-based anorexia in female mice. 16 running wheels (Columbus instruments) are available.
Running wheels; room 334 (BioHazard)
Low profile, wireless running wheels for mice. These running wheels fit the metabolic cages and may be used as stand alone or in combination with a metabolic cages experiment. As the vertical running wheels, the wheels may serve as a way for assessing general locomotion, circadian activity and may used to induce activity-based anorexia in female mice. 8 running wheels (Med Associates) are available.
More information can be found in the manual.
Body Temperature; (portable)
Body temperature is measured by subcutaneous implantable microchips (BMDS) that measure temp accurately (±0.2°C) and are easily detected by an external reader. Rectal thermometers are available as well.
Hyperinsulinemic-euglycemic clamp; room 306
Glucose clamp is the gold standard for assessing insulin sensitivity. Hyperinsulinemic-euglycemic clamp can currently be done for assessment of glucose infusion rate (GIR) that correlates with insulin sensitivity. Relevant tissues may be collected at the end of the clamp.