IVIS SPECTRUM, Whole Animal Bioluminescence and Fluorescence Imaging
Lorry Lokey Facility room 315
The IVIS Spectrum is the newest imaging system available from Caliper Life Sciences. Like previous IVIS systems, it uses an original Xenogen’s novel patented optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals.
The IVIS Spectrum is one of the most versatile and advanced in vivo imaging systems available on the market today. An optimized set of high efficiency filters and spectral un-mixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. It also offers single-view 3D tomography for both fluorescent and bioluminescent reporters that can be analysed in an anatomical context using our Digital Mouse Atlas.
For advanced fluorescence imaging, the IVIS Spectrum has the capability to use either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate in vivo fluorescent sources. 3D diffuse fluorescence tomography can be performed to determine source localization and concentration using the combination of structured light and trans illumination fluorescent images. The instrument is equipped with 10 narrow band excitation filters (30nm bandwidth) and 18 narrow band emission filters (20nm bandwidth) that assist in significantly reducing autofluorescence by the spectral scanning of filters and the use of spectral unmixing algorithms. In addition, the spectral unmixing tools allow the researcher to separate signals from multiple fluorescent reporters within the same animal.
IVIS KINETIC, Whole Animal Bioluminescence and Fluorescence Imaging
Lorry Lokey Facility room 339 (Biohazard)
The IVIS Kinetic from Caliper Life Sciences provides a real time, fast imaging system-enabling acquisition of biologically relevant events within milliseconds. The IVIS Kinetic can perform both quantitative luminescence and fluorescence as a standard high signal to noise imager and as a high-speed imager. The system includes a highly sensitive EMCCD camera for signal enhancement and the ability to reduce exposure times enabling fast kinetics. The IVIS Kinetic offers a light-tight injection port which supporting a syringe injector system enabling real time compound and/or substrate administration. A removable pull-out tray is available to facilitate pre-imaging animal procedures.
IVIS Lumina XRMS In Vivo Imaging System
Lorry Lokey Facility room 308
Industry leading Fluorescence, Bioluminescence and X-Ray Imaging - All-In-One benchtop instrument! The IVIS® Lumina XRMS (x-ray multi-species optical imaging system) adds to the versatility of the IVIS Lumina XR by offering the flexibility to image rodents with precise optical and X-Ray overlay. The IVIS Lumina XRMS Series is capable of imaging all common fluorescent and bioluminescent reporters or dyes. The system is equipped with all range of filter sets to image reporters that emit from green to near-infrared. High resolution, sharp cut-off filters are simply interchangeable to achieve the highest performance, sensitivity and spectral unmixing. Living Image software yields high-quality, reproducible, quantitative results incorporating instrument calibration, background subtraction and the image algorithms. Simple user guided spectral unmixing allows detection and separation of multiple reporters, and Living Image provides the precise overlay to see your optical reporters together with anatomical surface or X-ray features.
Zeiss LSM 880 NLO-OPO, Advanced Multi Photon Microscopy
Lorry Lokey Facility room 308
ZEISS LSM 880 NLO OPO is a state of the art microscopy imaging system specifically dedicated for the advanced in vivo applications.
The new microscope has numbers of GaAsP detectors, dedicated to use for increased resolution, increased signal to noise ratio, increased acquisition speed, or any combination of these improvements.
The microscope equipped with a new Chameleon MPX laser (Coherent, USA) for multi photon fluorescent excitation of biological samples as well as Second Harmonic Generation imaging (SHG) of deep tissue inside live animals. This laser has a unique unit, called Optical Parametric Oscillator (OPO). OPO allows tuning the laser in a range from 680 - 1300 nm. The use of longer wavelength (1200 -1300 nm) provides a critical advantage over standard multi-photon microscope, allowing image more efficiently of red shifted fluorescent proteins. OPO capabilities are unique for Israel (there is no such system elsewhere in Israel).
Overall our new microscope is dedicated for confocal and multi-photon imaging, including parallel spectral imaging, harmonics generation, and detection of red fluorescent proteins. The system features a novel scale method for extra-deep (up to 5. 6mm) imaging. It will be particularly useful for whole brain imaging. It is also capable of doing Fluorescence Correlation Spectroscopy using internal GaAsP multichannel detector as well as to measure fluorescence polarization anisotropy. In addition the system will be equipped with FLIM equipment and optics (which was already purchased) for fluorescence lifetime measurements. FLIM imaging is particularly useful in neurons, where light scattering by brain tissue is problematic for ratiometric imaging.
Multi Spectral Opto-acoustic Tomography (MSOT), ITheraMedical Optoacoustic Thomography
Lorry Lokey Facility room 310
Technical information for MSOT inVision system:
Single-wavelength optoacoustic imaging at 10 Hz frame rate
Real-time spectral component visualization at up to 5 Hz frame rate
Penetration depth of 2-4 cm, sufficient for whole-body small animal imaging
Cross-sectional spatial in-plane resolution: 150 μm
High-energy / fast-tunable laser system (100 mJ / 10 ms)
Tomographic ultrasound detector array with 256 elements
Image acquisition fully automated
Data post-processing suite for spectral and temporal analysis
Olympus SZX-RFL2, Intravital Microscopy and Image guided microsurgery
Lorry Lokey Facility room 306
The SZX-RFL2 Zoom Stereo Microscope gives the user maximum flexibility in observing the specimen without switching objectives. A series of parfocal objectives is also available along with a dual nosepiece. This allows the user to utilize the full zoom range of two objectives by easily rotating one or the other into the light path. Using the 0.5x and 1x objectives and 10x eyepieces on the SZX gives an effective zoom range of 3.5x to 63x.
SZX-RFL2 - Illuminator with 4-position slider for brightfield and fluorescence filter sets. Fluorescence excitation is accomplished via dichromatic mirrors in the optical axes of the microscope. The microscope objective acts as both the illuminating and imaging lens.
SZX-RFL2 is Equipped with 100W mercury lamp for fluorescent applications. It has 2 Camera Ports.
Additional accessories Illumination:
- Near Infrared Filter (NIR) set for Schott KL 1500 LCD light source for NIR fluorescent applications.
- Laser assemble Elfi-C for Dynamic Light Scattering Imaging
Olympus MVX, Intravital Microscopy and Image guided microsurgery; room 306
The perfect microscope for fluorescence observation of intact organisms must combine maximum detection efficiency from macro−to−micro magnifications with a high zoom and high NA for resolution of fine details. The MVX10 MacroView brings these factors together with low autofluorescence and other unique features to bridge the gap between macro and micro observation and providing unprecedented brightness, resolution and precision to the imaging system.