Polymerase Chain Reaction (PCR) instruments

Instrument Specification

The unit has several PCR apparatuses: a SensoQuest LabCycler 96 Gradient Gold an Eppendorf Mastercycler Gradient and an Eppendorf Mastercycler Personal. PCR amplifies exact DNA sequences. It can also be used to create specific changes in DNA sequences (site directed mutagenesis) and insert DNA sequences into specific sites (restriction free cloning). A Real Time or quantitative PCR instrument (Applied Biosystems StepOnePlus) allows quantification of DNA template in the PCR mixture. Differential RNA/DNA expression is done by following in real time DNA amplification.


Perlman Chemical Sciences Building Room 10

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