Integrated Calendar

Cells transduce information across their plasma membrane via engagement of surface receptors and the subsequent recruitment of intracellular proteins to these receptors. Such receptor-regulated cellular signaling often results in the formation of transient, heterogeneous protein complexes and macro-molecular clusters that serve to amplify, relay and regulate the incoming signals. Important examples include signaling complexes downstream of receptor tyrosine kinases (RTKs), immune receptors and G protein-coupled receptors (GPCRs). Although these signaling structures play a crucial role in health and disease, including immune activation, cancer, and HIV infection, the detailed biophysical mechanisms by which they assemble and serve to activate cells remain poorly understood due to shortcomings in current research techniques.
We have developed and applied two-color photoactivated localization microscopy (PALM) to study the organization, structure and function of signaling complexes in the activation of immune (T) cells, at the single molecule level. Using this method, individual molecules can be identified and localized with resolution down to ~20nm. Second-order and clustering statistics were applied to resolve size-distributions of signaling complexes and molecular interactions at the plasma membrane of the cells. Our results show that nanoclusters, signaling complexes as small as dimers and trimers, govern the earliest events of T cell activation through dynamic interactions. Importantly, we show that these signaling complexes assume, in several ways, novel patterns of nanoscale organization that are crucial for cell activation. Taking a multidisciplinary approach, including the use of genetic mutagenesis, targeted drugs, statistical analysis and Monte-Carlo simulations, we could trace mechanisms that govern the formation of these patterns. Such mechanisms include combinations of dynamic protein-protein and protein-lipid interactions, hop-diffusion, confinement and patterning by the cell cytoskeleton and plasma membrane. These results extend our understanding of the mechanism of immune cell activation, findings also relevant to other receptor systems. I will further discuss novel research techniques that we have developed to better study critical signaling pathways in live interacting cells and in molecular detail.