Integrated Calendar

Malaria is the most serious mosquito-borne parasitic disease, caused mainly by the intracellular parasite Plasmodium falciparum. This parasite invades human red blood cells and releases extracellular vesicles (EVs) to alter its host responses. It becomes clear that EVs are generally composed of sub-populations. Seeking to identify the various EV subpopulations we subjected Pf-EVs to size-separation analysis. Multi-technique analysis revealed two distinct EV subpopulations differing in size, protein content, membrane packing and fusion capabilities. Remarkably, the small EVs fuse to early-endosome conditions at significantly greater levels than the large EVs, suggesting different destinations.
Moreover, we surprisingly found that upon Pf-EV internalization into monocytes, three parasitic transcripts are transferred into the host cell’s nucleus. These findings open a new direction of investigation for understanding the role of Pf-EVs on the human host.

This colloquium will be divided into two applications parts, dealing with synthesis of supported molecular catalysts and solid catalysts for photoprotection. In the first of these areas, we describe a mechanical approach for stabilizing supported weakly interacting active sites (i.e. those that interact non-covalently with the support) against aggregation and coalescence. We use silica as a prototypical example of a support, and an iridium pair-site catalyst incorporating bridging calixarene ligands as an active site. Atomic-resolution imaging of the Ir centers before and after ethylene-hydrogenation catalysis show the metals resisted aggregation and deactivation, remaining atomically dispersed and accessible for catalysis. When active sites are located at unconfined environments, the rate constants for ethylene hydrogenation are markedly lower compared with confining external-surface pockets [1], in line with prior observations of similar effects in olefin epoxidation catalysis [2,3]. Altogether, these examples represent new opportunities for enhancing reactivity on surfaces by synthetically controlling mechanical features of active site catalyst environments.

In the second of these areas, reactive oxygen species (ROS) are associated with several human health pathologies and are invoked in the degradation of natural ecosystems as well as building materials that are used in modern infrastructure (e.g., paints and coatings, polymers, etc). Natural antioxidants such as vitamin E function as stoichiometric reductants (i.e. reaction with ROS synthesizes rancid oils). While enzymes such as superoxide dismutase working in tandem with catalase decompose decompose ROS to H2O and O2 through H2O2 as an intermediate, these enzymes are fragile and costly. Other non-stoichiometric commercial antioxidants that degrade ROS include hindered amine light stabilizers (HALS). Here, we demonstrate that cerium carbonate acts as a degradation catalyst for photogenerated ROS, and describe the performance and characterization of this new catalyst using X-ray photoelectron spectroscopy, and in comparison with HALS and stoichiometric reductants. Our results demonstrate catalytic antioxidant activity of cerium carbonate when dispersed in polymethylmethacrylate polymer. FTIR data demonstrate that a dispersion of 2 wt. % cerium carbonate within the polymer essentially stops degradation by photogenerated ROS, which otherwise cause oxidation of the polymer backbone, in the control polymer lacking cerium carbonate. Experiments with methylene blue dye in aqueous solution demonstrate that cerium carbonate decreases the rate of ROS degradation of dye, in the presence of UV irradiation and air by 16 fold. These effects become even more pronounced (over 600 fold decrease in rate of ROS dye degradation) when cerium carbonate is paired with a photoactive metal oxide. The mechanism involved in this latter case crudely mimics the enzyme tandem sequence referred to above.

[1] C. Schöttle, E. Guan, A. Okrut, N. A. Grosso-Giordano, A. Palermo, A. Solovyov, B. C. Gates, A. Katz*, Journal of the American Chemical Society, J. Am. Chem. Soc. 2019, 141, 4010-4015.
[2] N. A. Grosso-Giordano, C. Schroeder, A. Okrut, A. Solovyov, C. Schottle, W. Chasse, N. Marinkoyic, H. Koller, S. I. Zones, A. Katz, Journal of the American Chemical Society 2018, 140, 4956-4960.
[3] N. A. Grosso-Giordano, A. S. Hoffman, A. Boubnov, D. W. Small, S. R. Bare, S. I. Zones, A. Katz, Journal of the American Chemical Society 2019, 141, 7090-7106.
[4] M. K. Mishra, J. Callejas, M. Pacholski, J. Ciston, A. Okrut, A. Van Dyk, D. Barton, J. C. Bohling, A. Katz, ACS Applied Nano Materials 2021, 4, 11, 11590-11600.

The Legacy Survey of Space and Time (LSST), the first project to be undertaken
at the new Vera Rubin Observatory, will be the most comprehensive optical astronomical
survey ever undertaken. Starting in 2024, Rubin Observatory will obtain panoramic images
covering the sky visible from its location in Chile every clear night for ten years.
The resulting hundreds of petabytes of imaging data, essentially a digital color movie
of the night sky, will include about 40 billion stars and galaxies, and will be used for investigations ranging from cataloging dangerous near-Earth asteroids to fundamental
physics such as characterization of dark matter and dark energy.

I will start my presentation with an overview of LSST science drivers and system design,
and continue with a construction status report for the Vera Rubin Observatory. I will
conclude with a brief discussion of a few Big Data challenges that need to be addressed
before LSST data can be used for precise cosmological measurements.

Our ability to correctly diagnose and treat mental illness is limited by the overlap in symptoms of many disorders, despite differing etiology. Determining the proper course of treatment is quite difficult because treating individual symptoms does not always lead to successful remission and typically involves a trial-and-error approach. Task-based functional MRI has become a highly useful tool for determining the brain regions involved in cognition and behavior in humans, with the potential to be used to find biomarkers of mental illness and treatment outcomes. Much of the research in this domain has focused on the differences in brain activation between groups of individuals with specific mental disorders and typically developing “control” groups. However, by relating brain activation patterns of clinical groups to symptom severity, developmental processes, and response to treatment at the individual level, we can determine brain-based markers that have the potential to be used as diagnostic tools in the future and to determine whether certain treatments would be helpful based on specific brain activation patterns. In this talk, I will present data from studies using task-based functional MRI in autism spectrum disorder, schizophrenia, and childhood adversity that illustrate the potential of this technology for diagnostic and treatment purposes. I will also discuss the promises and limitations of using fMRI as a clinical tool.

It's been exactly 10 years since the Higgs Boson Discovery (July 4th, 2012). The Higgs Boson discovery is the biggest achievement of the Large Hadron Collider (LHC) at CERN, and one of the milestones of experimental Particle Physics. We will describe the road to the Higgs Boson discovery, its importance, and the status of the measurement of its properties since its discovery.