Integrated Calendar

Zoom Link: https://weizmann.zoom.us/j/94661424796?pwd=U0Z1YjdsbGUrV29STEZlMVhweUtXUT09




All our cells contain the same genetic information, encoded in the sequence of nucleotides that compose our DNA. The identity of different cells, and their response to different stimuli, is therefore controlled by processes regulating which subset of genes is “expressed” at a specific cell and a specific time. The first step in gene expression regulation is the binding of a special family of proteins, called transcription factors, to specific sequences in regulatory regions in the DNA. Packaging of the DNA into the dense structure of chromatin, and chemical modifications of the DNA, provide the cell with the possibility of dynamically modulating expression but add additional layers of complexity to the process in ways that are not fully understood. In my talk, I will report on our work using single-molecule optical tweezers assays to study how the thermodynamics and kinetics of transcription factor binding are modulated by these different layers of information.
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Analysis of intact proteins by native mass spectrometry has emerged as a powerful tool for obtaining insight into subunit diversity, post-translational modifications, stoichiometry, structural arrangement, stability, and overall architecture. Typically, such an analysis is performed following protein purification procedures, which are time consuming, costly, and labor intensive. As this technology continues to move forward, advances in sample handling and instrumentation have enabled the investigation of intact proteins in crude samples, offering rapid analysis and improved conservation of the biological context. This emerging approach is expected to impact many scientific fields, including biotechnology, pharmaceuticals, and clinical sciences. In my talk I will discuss the information that can be retrieved by such experiments as well as the applicability of the method by presenting the characterization of engineered proteins, drug binding, antibody specificity and protein-protein interactions.

Zoom link to join:
https://weizmann.zoom.us/j/96608033618?pwd=SEdJUkR2ZzRBZ3laUUdGbWR1VFJTdz09

Meeting ID: 966 0803 3618
Password: 564068

Host: Dr. Rita Schmidt rita.schmidt@weizmann.ac.il tel: 9070