לוח שנה משולב

Alternative promoter usage contributes to the complexity of genome-encoded transcripts but little is known about its impact on translation. To obtain a global view of transcription start site (TSS) selection effect on translation we performed TSS mapping of the translatome under normal growth condition and following energy stress. Our findings uncovered several levels of coordination of transcription and translation. In this talk I will present the data analysis of these experiments along with in-depth inquiries of several intriguing examples.

Abstract (form the eLife paper:) ): Insertion of helix-forming segments into the membrane and their association determines the structure, function, and expression levels of all plasma membrane proteins. However, systematic and reliable quantification of membrane-protein energetics has been challenging. We developed a deep mutational scanning method to monitor the effects of hundreds of point mutations on helix insertion and self-association within the bacterial inner membrane. The assay quantifies insertion energetics for all natural amino acids at 27 positions across the membrane, revealing that the hydrophobicity of biological membranes is significantly higher than appreciated. We further quantitate the contributions to membrane-protein insertion from positively charged residues at the cytoplasm-membrane interface and reveal large and unanticipated differences among these residues. Finally, we derive comprehensive mutational landscapes in the membrane domains of Glycophorin A and the ErbB2 oncogene, and find that insertion and self-association are strongly coupled in receptor homodimers.

Recent experimental results from the LHC have placed strong constraints on the masses of colored superpartners. The MSSM parameter space is also constrained by the measurement of the Higgs boson mass, and the requirement that the relic density of lightest neutralinos be consistent with observations. Although large regions of the MSSM parameter space can be excluded by these combined bounds, leptophilic versions of the MSSM can survive these constraints. We consider a scenario in which the requirements of minimal flavor violation, vanishing CP-violation, and mass universality are relaxed, specifically focusing on scenarios with light sleptons. We find a large region of parameter space, analogous to the original bulk region, for which the lightest neutralino is a thermal relic with an abundance consistent with that of dark matter. We find that these leptophilic models are constrained by measurements of the magnetic and electric dipole moments of the electron and muon, and that these models have interesting signatures at a variety of indirect detection experiments. We also consider a related scenario in which dark matter is bino-like and dark matter-nucleon spin-independent scattering occurs via the exchange of light squarks which exhibit left-right mixing. We show that direct detection experiments such as LUX and SuperCDMS will be sensitive to a wide class of such models through spin-independent scattering. Moreover, these models exhibit properties, such as isospin violation, that are not typically observed for the MSSM LSP if scattering occurs primarily through Higgs exchange. The dominant nuclear physics uncertainty is the quark content of the nucleon, particularly the strangeness content.

The discovery of topological band insulators is one of the most exciting developments in condensed matter physics in the last decade. In this talk I will describe how insights from the study of the relatively simple topological insulators are revolutionizing our theoretical understanding of more complex quantum many body systems. The latter include states of strongly interacting quantum matter which have been at the forefront of research in the last 3 decades. Specific examples include quantum spin liquid states of insulating magnets, and the theory of composite fermions in quantum Hall systems.
The theory of topological insulators unifies these seemingly diverse problems, and provides a new window through which to view them leading to many new and fundamental results.