לוח שנה משולב

Understanding the function of biomolecules on a molecular level requires knowledge about their structure and conformational changes during function. Site directed spin labeling (SDSL) in combination with pulsed dipolar EPR spectroscopy (PDS) enables to gather such information on the nanometer length scale. In the talk, it will be shown that this approach enables the localization of metal ions within the fold of biomolecules, also of those metal ions with large zero-field splitting, where the high-field approximation breaks down. It will also be shown that this cannot only be done in vitro but also within cells. Last but not least, an example will be given where a conformational change of a protein is not only followed on the length- but also on the microsecond time resolution using PDS/SDSL.