Protein Purification Publications

  1. Weinreb E., McBride J. M., Siek M., Rougemont J., Renault R., Peleg Y., Unger T., Albeck S., Fridmann-Sirkis Y., Lushchekina S., Sussman J. L., Grzybowski B. A., Zocchi G., Eckmann J. P., Moses E. & Tlusty T. (2025). Enzymes as viscoelastic catalytic machines.  Nature Physics. 2025 Mar 28 , .
  2. Osher E., Anis Y., Singer-Shapiro R., Urshanski N., Unger T., Albeck S., Bogin O., Weisinger G., Kohen F., Valevski A., Fattal-Valevski A., Sagi L., Weitman M., Shenberger Y., Sagiv N., Navon R., Wilchek M. & Stern N. (2024). Treating late-onset Tay Sachs disease: Brain delivery with a dual trojan horse protein.  Molecular Therapy Methods and Clinical Development. 2024 Sept 12 , 32 (3).
  3. Halfin O., Avram L., Albeck S., Unger T., Motiei L. & Margulies D. (2024). Unnatural Enzyme Activation by a Metal-Responsive Regulatory Protein.  Chemical Science. 2024 Sept 21 , 15 (35):14209-14217.
  4. Lal M., Kesselman E., Wachtel E., Kleinerman O., Peleg Y., Albeck S., Majhi K., Sheves M. & Patchornik G. (2024). His-tag based supramolecular biopolymerization.  Scientific Reports. 2024 Nov 16 , 14 .
  5. Pokorna S., Khersonsky O., Lipsh-Sokolik R., Goldenzweig A., Nielsen R., Ashani Y., Peleg Y., Unger T., Albeck S., Dym O., Tirosh A., Tarayra R., Hocquemiller M., Laufer R., Ben-Dor S., Silman I., Sussman J. L., Fleishman S. J. & Futerman A. H. (2023). Design of a stable human acid-β-glucosidase: towards improved Gaucher disease therapy and mutation classification.  FEBS Journal. 2023 Jul , 290 (13):3383-3399.
  6. Franz F., Tapia-Rojo R., Winograd-Katz S., Boujemaa-Paterski R., Li W., Unger T., Albeck S., Aponte-Santamaria C., Garcia-Manyes S., Medalia O., Geiger B. & Gräter F. (2023). Allosteric activation of vinculin by talin.  Nature Communications. 2023 Jul 18 , 14 (1).
  7. Gould N. L., Scherer G. R., Carvalho S., Shurrush K., Kayyal H., Edry E., Elkobi A., David O., Foqara M., Thakar D., Pavesi T., Sharma V., Walker M., Maitland M., Dym O., Albeck S., Peleg Y., Germain N., Babaev I., Sharir H., Lalzar M., Shklyar B., Hazut N., Khamaisy M., Lévesque M., Lajoie G., Avoli M., Amitai G., Lefker B., Subramanyam C., Shilton B., Barr H. & Rosenblum K. (2023). Specific quinone reductase 2 inhibitors reduce metabolic burden and reverse Alzheimers disease phenotype in mice.  Journal of Clinical Investigation. 2023 Oct 2 , 133 (19).
  8. Dym O., Aggarwal N., Ashani Y., Leader H., Albeck S., Unger T., Hamer-Rogotner S., Silman I., Tawfik D. S. & Sussman J. L. (2023). The impact of molecular variants, crystallization conditions and the space group on ligand-protein complexes: a case study on bacterial phosphotriesterase.  Acta Crystallographica Section D: Structural Biology. 2023 Nov , 79 (Pt 11):992-1009.
  9. Goldsmith M., Barad S., Peleg Y., Albeck S., Dym O., Brandis A., Mehlman T. & Reich Z. (2022). The identification and characterization of an oxalyl-CoA synthetase from grass pea ( Lathyrus sativus L.).  RSC Chemical Biology. 2022 Mar 9 , 3 (3):320-333.
  10. Allouche-Arnon H., Khersonsky O., Tirukoti N. D., Peleg Y., Dym O., Albeck S., Brandis A., Mehlman T., Avram L., Harris T., Yadav N. N., Fleishman S. J. & Bar-Shir A. (2022). Computationally designed dual-color MRI reporters for noninvasive imaging of transgene expression.  Nature biotechnology. 2022 Jul , 40 (7):1143-1149.
  11. Goldsmith M., Barad S., Knafo M., Savidor A., Ben-Dor S., Brandis A., Mehlman T., Peleg Y., Albeck S., Dym O., Ben-Zeev E., Barbole R. S., Aharoni A. & Reich Z. (2022). Identification and characterization of the key enzyme in the biosynthesis of the neurotoxin β-ODAP in grass pea.  The journal of Biological chemistry. 2022 May 1 , 298 (5).
  12. Seal M., Jash C., Jacob R., Feintuch A., Harel Y., Albeck S., Unger T. & Goldfarb D. (2021). Evolution of CPEB4 Dynamics across its Liquid-Liquid Phase Separation Transition.  Journal Of Physical Chemistry B. 2021 Dec 2 , 125 (47):12947-12957.
  13. Peleg Y., Vincentelli R., Collins B. M., Chen K., Livingstone E. K., Weeratunga S., Leneva N., Guo Q., Remans K., Perez K., Bjerga G. E., Larsen Ø., Vaněk O., Skořepa O., Jacquemin S., Poterszman A., Kjaer S., Christodoulou E., Albeck S., Dym O., Ainbinder E., Unger T., Schuetz A., Matthes S., Bader M., de Marco A., Storici P., Semrau M. S., Stolt-Bergner P., Aigner C., Suppmann S., Goldenzweig A. & Fleishman S. J. (2021). Community-Wide Experimental Evaluation of the PROSS Stability-Design Method.  Journal of Molecular Biology. 2021 Jun 25 , 433 (13).
  14. Davidi D., Shamshoum M., Guo Z., BarOn Y. M., Prywes N., Oz A., Jablonska J., Flamholz A., Wernick D. G., Antonovsky N., Pins B., Shachar L., Hochhauser D., Peleg Y., Albeck S., Sharon I., MuellerCajar O. & Milo R. (2020). Highly active rubiscos discovered by systematic interrogation of natural sequence diversity.  The EMBO Journal. 2020 Sept 15 , 39 (18).
  15. Giannoulis A., Feintuch A., Barak Y., Mazal H., Albeck S., Unger T., Yang F., Su X. & Goldfarb D. (2020). Two closed ATP- and ADP-dependent conformations in yeast Hsp90 chaperone detected by Mn(II) EPR spectroscopic techniques.  Proceedings of the National Academy of Sciences of the United States of America. 2020 Jan 7 , 117 (1):395-404.
  16. Gabizon R., Shraga A., Gehrtz P., Livnah E., Shorer Y., Gurwicz N., Avram L., Unger T., Aharoni H., Albeck S., Brandis A., Shulman Z., Katz B., Herishanu Y. & London N. (2020). Efficient targeted degradation via reversible and irreversible covalent PROTACs.  Journal of the American Chemical Society. 2020 Jul 8 , 142 (27):11734-11742.
  17. Warszawski S., Katz A. B., Lipsh R., Khmelnitsky L., Nissan G. B., Javitt G., Dym O., Unger T., Knop O., Albeck S., Diskin R., Fass D., Sharon M. & Fleishman S. J. (2020). Erratum: Optimizing antibody affinity and stability by the automated design of the variable light-heavy chain interfaces (PLoS Computational Biology(2019)15: 8(e1007207)Doi: 10.1371/journal.pcbi.1007207).  PLoS Computational Biology. 2020 Oct 21 , 16 (10).
  18. Dalaloyan A., Martorana A., Barak Y., Gataulin D., Reuveny E., Howe A., Elbaum M., Albeck S., Unger T., Frydman V., Abdelkader E. H., Otting G. & Goldfarb D. (2019). Tracking Conformational Changes in Calmodulin in vitro, in Cell Extract, and in Cells by Electron Paramagnetic Resonance Distance Measurements.  ChemPhysChem. 2019 Jul 16 , 20 (14):1860-1868.
  19. Shraga A., Olshvang E., Davidzohn N., Khoshkenar P., Germain N., Shurrush K., Carvalho S., Avram L., Albeck S., Unger T., Lefker B., Subramanyam C., Hudkins R. L., Mitchell A., Shulman Z., Kinoshita T. & London N. (2019). Covalent Docking Identifies a Potent and Selective MKK7 Inhibitor.  Cell Chemical Biology. 2019 Jan 17 , 26 (1):98 - 108.
  20. Warszawski S., Katz A. B., Lipsh R., Khmelnitsky L., Ben Nissan G., Javitt G., Dym O., Unger T., Knop O., Albeck S., Diskin R., Fass D., Sharon M. & Fleishman S. J. (2019). Optimizing antibody affinity and stability by the automated design of the variable light-heavy chain interfaces.  PLoS Computational Biology. 2019 Aug 23 , 15 (8).