Protein Expression Publications

  1. Weinreb E., McBride J. M., Siek M., Rougemont J., Renault R., Peleg Y., Unger T., Albeck S., Fridmann-Sirkis Y., Lushchekina S., Sussman J. L., Grzybowski B. A., Zocchi G., Eckmann J. P., Moses E. & Tlusty T. (2025). Enzymes as viscoelastic catalytic machines.  Nature Physics. 2025 Mar 28 , 21 (5):787-798.
  2. Prasad P. K., Toraskar S., Khan S., Granot T., Sirkis Y. F., Yardeni E. H., Albeck S., Unger T., Petrovich-Kopitman E., Addadi Y., Raigawali R., Anand S., Vishweshwara S. S., Shanthamurthy C. D., Oppenheimer-Low N., Kikkeri R., Avinoam O., Motiei L. & Margulies D. (2025). Low-Background His-Tag-Targeting Probes for Turn-On Fluorescence Detection of Cell Surface Proteins and Their Binding Interactions.  Small. 2025 Jul 4 , 21 (33).
  3. Peer L., Dym O., Elad N., Tirosh A., Jacobovitch J., Sivan E., Angel M., Albeck S., Schuldiner M., Peleg Y. & Zalckvar E. (2025). A cryo-electron microscopy structure of yeast Pex5 in complex with a cargo uncovers a novel binding interface.  Journal of Cell Science. 2025 Jun , 138 (12).
  4. Razi S. G., Withanage T. J., Krichevsky O., Wachtel E., Peleg Y., Albeck S. & Patchornik G. (2025). Fluorescent supramolecular fibers and sheets built from doubly hexa-His tagged mCherry proteins conjugated by divalent metal cations.  International Journal of Biological Macromolecules. 2025 Oct , 327 .
  5. Yado S., Zoabi R., Brezinger-Dayan K., Albeck S., Unger T., Meiron M., Eisenberg G., Nahmad A. D., Gilat A. T., Besser M. J. & Geiger B. (2025). Differential effects of immobilized CCL21 and ICAM1 on TILs with distinct expansion properties.  Frontiers in Immunology. 2025 Oct 3 , 16 .
  6. Weinreb E., McBride J. M., Siek M., Rougemont J., Renault R., Peleg Y., Unger T., Albeck S., Fridmann-Sirkis Y., Lushchekina S., Sussman J. L., Grzybowski B. A., Zocchi G., Eckmann J. P., Moses E. & Tlusty T. (2025). Enzymes as viscoelastic catalytic machines.  Nature Physics. 2025 Mar 28 , 21 (5):787-798.
  7. Feigelson S. W., Dadosh T., Levi N., Sapoznikov A., Weinstein-Marom H., Blokon-Kogan D., Avraham Y., Unger T., Gross G., Dahan R. & Alon R. (2025). CD32B1, a versatile non-signaling antibody-binding scaffold for enhanced T cell adhesion to tumor stromal cognate antigens.  Frontiers in Immunology. 2025 Feb 10 , 16 .
  8. Halfin O., Avram L., Albeck S., Unger T., Motiei L. & Margulies D. (2024). Unnatural Enzyme Activation by a Metal-Responsive Regulatory Protein.  Chemical Science. 2024 Sept 21 , 15 (35):14209-14217.
  9. Oranges M., Giannoulis A., Vanyushkina A., Sirkis Y. F., Dalaloyan A., Unger T., Su X. C., Sharon M. & Goldfarb D. (2024). C-terminal domain dimerization in yeast Hsp90 is moderately modulated by the other domains.  Biophysical Journal. 2024 Jan 16 , 123 (2):172-183.
  10. Quinn S., Zhang N., Fenton T. A., Brusel M., Muruganandam P., Peleg Y., Giladi M., Haitin Y., Lerche H., Bassan H., Liu Y., Ben-Shalom R. & Rubinstein M. (2024). Complex biophysical changes and reduced neuronal firing in an SCN8A variant associated with developmental delay and epilepsy.  Biochimica et Biophysica Acta - Molecular Basis of Disease. 2024 Jun , 1870 (5).
  11. Sen S., Bhowmik P., Tiwari S., Peleg Y. & Bandyopadhyay B. (2024). Versatility of reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) from diagnosis of early pathological infection to mutation detection in organisms.  Molecular Biology Reports. 2024 Jan 25 , 51 (1).
  12. Berman P., de Haro L. A., Cavaco A. R., Panda S., Dong Y., Kuzmich N., Lichtenstein G., Peleg Y., Harat H., Jozwiak A., Cai J., Heinig U., Meir S., Rogachev I. & Aharoni A. (2024). The biosynthetic pathway of the hallucinogen mescaline and its heterologous reconstruction.  Molecular Plant. 2024 Jul 1 , 17 (7):1129-1150.
  13. Lal M., Kesselman E., Wachtel E., Kleinerman O., Peleg Y., Albeck S., Majhi K., Sheves M. & Patchornik G. (2024). His-tag based supramolecular biopolymerization.  Scientific Reports. 2024 Nov 16 , 14 .
  14. Osher E., Anis Y., Singer-Shapiro R., Urshanski N., Unger T., Albeck S., Bogin O., Weisinger G., Kohen F., Valevski A., Fattal-Valevski A., Sagi L., Weitman M., Shenberger Y., Sagiv N., Navon R., Wilchek M. & Stern N. (2024). Treating late-onset Tay Sachs disease: Brain delivery with a dual trojan horse protein.  Molecular Therapy Methods and Clinical Development. 2024 Sept 12 , 32 (3).
  15. Peri L., Matzov D., Huxley D. R., Rainish A., Fierro F., Sapir L., Pfeiffer T., Waterloo L., Hübner H., Peleg Y., Gmeiner P., McCormick P. J., Weikert D., Niv M. Y. & Shalev-Benami M. (2024). A bitter anti-inflammatory drug binds at two distinct sites of a human bitter taste GPCR.  Nature Communications. 2024 Nov 18 , 15 .
  16. Goldsmith M., Avram L., Brandis A., Mehlman T., Ben-Dor S., Peleg Y., Ben-Zeev E., Barbole R. S. & Reich Z. (2024). LsBOS utilizes oxalyl-CoA produced by LsAAE3 to synthesize β-ODAP in grass pea.  Nature Communications. 2024 Aug 8 , 15 .
  17. Herynek Š., Svoboda J., Huličiak M., Peleg Y., Škultétyová Ľ., Mikulecký P. & Schneider B. (2024). Increasing recombinant protein production in E. coli via FACS-based selection of N-terminal coding DNA libraries.  FEBS Journal. 2024 Dec 26 , 292 (5):1070-1085.
  18. Schütz A., Bernhard F., Berrow N., Buyel J. F., Ferreira-da-Silva F., Haustraete J., van den Heuvel J., Hoffmann J. E., de Marco A., Peleg Y., Suppmann S., Unger T., Vanhoucke M., Witt S. & Remans K. (2023). A concise guide to choosing suitable gene expression systems for recombinant protein production.  STAR Protocols. 2023 Dec 15 , 4 (4).
  19. Khersonsky O., Goldsmith M., Zaretsky I., Hamer-Rogotner S., Dym O., Unger T., Yona M., Fridmann-Sirkis Y. & Fleishman S. J. (2023). Stable Mammalian Serum Albumins Designed for Bacterial Expression.  Journal of Molecular Biology. 2023 Sept 1 , 435 (17).
  20. Franz F., Tapia-Rojo R., Winograd-Katz S., Boujemaa-Paterski R., Li W., Unger T., Albeck S., Aponte-Santamaria C., Garcia-Manyes S., Medalia O., Geiger B. & Gräter F. (2023). Allosteric activation of vinculin by talin.  Nature Communications. 2023 Jul 18 , 14 (1).