The Advanced sequencing technologies  unit will serve as an advanced genomics technological laboratory that will allow Weizmann's scientists access to state-of-the-art genomic technologies.

The 'Sandbox' initiative will allow Weizmann scientists access to state-of-the-art technologies and protocols from advanced labs in the institute. The unit will introduce protocols by the form of 'hands-on' workshops, following which the user can independently use the Sandbox equipment and consumables.

Currently, we are running a workshop for special library preparation for RNA sequencing. The workshop will be a 3-day 'hands-on' exercise of the latest development of the 'bulk MARS-seq' protocol developed in Ido Amit lab. The protocol is designed for preparation of 3' RNA sequencing libraries from 1-50 ng of total RNA. In the workshop, each participant will learn to generate sequence-ready libraries starting from standard RNA samples using Sandbox equipment and consumables. Following the basic workshop training, the user will be able to independently use Sandbox equipment and consumables for library preparation of his/her own samples. Up to 96 libraries can be prepared simultaneously which can be sequenced in the unit's Nextseq desktop sequencer. Workshops are free of charge and limited to 3 participants. Workshops are open for Weizmann students and scientists only and take place periodically on an approximately bi-monthly schedule. Only workshop graduates will be able to use the sandbox facility.

To register for workshops or for further details, contact Dr. Hadas Keren-Shaul, hadask@weizmann.ac.il,  Tel 08-9346975.
Future workshops will include additional genomic technologies such as ChIP-seq and ATAC-seq.

Nextseq 500
The Nextseq 500 desktop sequencer provides the flexible power and simplicity for transcriptome, exome and whole genome sequencing in a single run. It is located in Levine building, second floor.

PacBio - Sequel
The PacBio Sequel sequencing system is built on Single molecule, Real-Time (SMRT) Sequencing and provides high consensus accuracy, uniform coverage and long read sequencing (http://www.pacb.com/products-and-services/pacbio-systems/sequel/).

10x Genomics
The 10x Genomics Chromium is based on GemCode technology and allows high throughput single cell 3' transcriptomics, genome or exome sequencing.