Protein Purification Publications

  1. Yado S., Zoabi R., Brezinger-Dayan K., Albeck S., Unger T., Meiron M., Eisenberg G., Nahmad A. D., Gilat A. T., Besser M. J. & Geiger B. (2025). Differential effects of immobilized CCL21 and ICAM1 on TILs with distinct expansion properties.  Frontiers in Immunology. 2025 Oct 3 , 16 .
  2. Razi S. G., Withanage T. J., Krichevsky O., Wachtel E., Peleg Y., Albeck S. & Patchornik G. (2025). Fluorescent supramolecular fibers and sheets built from doubly hexa-His tagged mCherry proteins conjugated by divalent metal cations.  International Journal of Biological Macromolecules. 2025 Oct , 327 .
  3. Weinreb E., McBride J. M., Siek M., Rougemont J., Renault R., Peleg Y., Unger T., Albeck S., Fridmann-Sirkis Y., Lushchekina S., Sussman J. L., Grzybowski B. A., Zocchi G., Eckmann J. P., Moses E. & Tlusty T. (2025). Enzymes as viscoelastic catalytic machines.  Nature Physics. 2025 Mar 28 , 21 (5):787-798.
  4. Peer L., Dym O., Elad N., Tirosh A., Jacobovitch J., Sivan E., Angel M., Albeck S., Schuldiner M., Peleg Y. & Zalckvar E. (2025). A cryo-electron microscopy structure of yeast Pex5 in complex with a cargo uncovers a novel binding interface.  Journal of Cell Science. 2025 Jun , 138 (12).
  5. Prasad P. K., Toraskar S., Khan S., Granot T., Sirkis Y. F., Yardeni E. H., Albeck S., Unger T., Petrovich-Kopitman E., Addadi Y., Raigawali R., Anand S., Vishweshwara S. S., Shanthamurthy C. D., Oppenheimer-Low N., Kikkeri R., Avinoam O., Motiei L. & Margulies D. (2025). Low-Background His-Tag-Targeting Probes for Turn-On Fluorescence Detection of Cell Surface Proteins and Their Binding Interactions.  Small. 2025 Jul 4 , 21 (33).
  6. Halfin O., Avram L., Albeck S., Unger T., Motiei L. & Margulies D. (2024). Unnatural Enzyme Activation by a Metal-Responsive Regulatory Protein.  Chemical Science. 2024 Sept 21 , 15 (35):14209-14217.
  7. Lal M., Kesselman E., Wachtel E., Kleinerman O., Peleg Y., Albeck S., Majhi K., Sheves M. & Patchornik G. (2024). His-tag based supramolecular biopolymerization.  Scientific Reports. 2024 Nov 16 , 14 .
  8. Osher E., Anis Y., Singer-Shapiro R., Urshanski N., Unger T., Albeck S., Bogin O., Weisinger G., Kohen F., Valevski A., Fattal-Valevski A., Sagi L., Weitman M., Shenberger Y., Sagiv N., Navon R., Wilchek M. & Stern N. (2024). Treating late-onset Tay Sachs disease: Brain delivery with a dual trojan horse protein.  Molecular Therapy Methods and Clinical Development. 2024 Sept 12 , 32 (3).
  9. Pokorna S., Khersonsky O., Lipsh-Sokolik R., Goldenzweig A., Nielsen R., Ashani Y., Peleg Y., Unger T., Albeck S., Dym O., Tirosh A., Tarayra R., Hocquemiller M., Laufer R., Ben-Dor S., Silman I., Sussman J. L., Fleishman S. J. & Futerman A. H. (2023). Design of a stable human acid-β-glucosidase: towards improved Gaucher disease therapy and mutation classification.  FEBS Journal. 2023 Jul , 290 (13):3383-3399.
  10. Franz F., Tapia-Rojo R., Winograd-Katz S., Boujemaa-Paterski R., Li W., Unger T., Albeck S., Aponte-Santamaria C., Garcia-Manyes S., Medalia O., Geiger B. & Gräter F. (2023). Allosteric activation of vinculin by talin.  Nature Communications. 2023 Jul 18 , 14 (1).
  11. Gould N. L., Scherer G. R., Carvalho S., Shurrush K., Kayyal H., Edry E., Elkobi A., David O., Foqara M., Thakar D., Pavesi T., Sharma V., Walker M., Maitland M., Dym O., Albeck S., Peleg Y., Germain N., Babaev I., Sharir H., Lalzar M., Shklyar B., Hazut N., Khamaisy M., Lévesque M., Lajoie G., Avoli M., Amitai G., Lefker B., Subramanyam C., Shilton B., Barr H. & Rosenblum K. (2023). Specific quinone reductase 2 inhibitors reduce metabolic burden and reverse Alzheimers disease phenotype in mice.  Journal of Clinical Investigation. 2023 Oct 2 , 133 (19).
  12. Dym O., Aggarwal N., Ashani Y., Leader H., Albeck S., Unger T., Hamer-Rogotner S., Silman I., Tawfik D. S. & Sussman J. L. (2023). The impact of molecular variants, crystallization conditions and the space group on ligand-protein complexes: a case study on bacterial phosphotriesterase.  Acta Crystallographica Section D: Structural Biology. 2023 Nov , 79 (Pt 11):992-1009.
  13. Goldsmith M., Barad S., Peleg Y., Albeck S., Dym O., Brandis A., Mehlman T. & Reich Z. (2022). The identification and characterization of an oxalyl-CoA synthetase from grass pea (Lathyrus sativus L.).  RSC Chemical Biology. 2022 Mar 1 , 3 (3):320-333.
  14. Allouche-Arnon H., Khersonsky O., Tirukoti N. D., Peleg Y., Dym O., Albeck S., Brandis A., Mehlman T., Avram L., Harris T., Yadav N. N., Fleishman S. J. & Bar-Shir A. (2022). Computationally designed dual-color MRI reporters for noninvasive imaging of transgene expression.  Nature Biotechnology. 2022 Jul , 40 (7):1143-1149.
  15. Goldsmith M., Barad S., Knafo M., Savidor A., Ben-Dor S., Brandis A., Mehlman T., Peleg Y., Albeck S., Dym O., Ben-Zeev E., Barbole R. S., Aharoni A. & Reich Z. (2022). Identification and characterization of the key enzyme in the biosynthesis of the neurotoxin β-ODAP in grass pea.  Journal of Biological Chemistry. 2022 May 1 , 298 (5).
  16. Franz F., Tapia-Rojo R., Winograd-Katz S., Boujemaa-Paterski R., Li W., Unger T., Albeck S., Aponte-Santamaria C., Garcia-Manyes S., Medalia O., Geiger B. & Gräter F. (2022). How talin allosterically activates vinculin.  BioRxiv. 2022 Aug 2 , .
  17. Seal M., Jash C., Jacob R., Feintuch A., Harel Y., Albeck S., Unger T. & Goldfarb D. (2021). Evolution of CPEB4 Dynamics across its Liquid-Liquid Phase Separation Transition.  Journal Of Physical Chemistry B. 2021 Dec 2 , 125 (47):12947-12957.
  18. Peleg Y., Vincentelli R., Collins B. M., Chen K., Livingstone E. K., Weeratunga S., Leneva N., Guo Q., Remans K., Perez K., Bjerga G. E., Larsen Ø., Vaněk O., Skořepa O., Jacquemin S., Poterszman A., Kjaer S., Christodoulou E., Albeck S., Dym O., Ainbinder E., Unger T., Schuetz A., Matthes S., Bader M., de Marco A., Storici P., Semrau M. S., Stolt-Bergner P., Aigner C., Suppmann S., Goldenzweig A. & Fleishman S. J. (2021). Community-Wide Experimental Evaluation of the PROSS Stability-Design Method.  Journal of Molecular Biology. 2021 Jun 25 , 433 (13).
  19. Gabizon R., Shraga A., Gehrtz P., Livnah E., Shorer Y., Gurwicz N., Avram L., Unger T., Aharoni H., Albeck S., Brandis A., Shulman Z., Katz B., Herishanu Y. & London N. (2020). Efficient targeted degradation via reversible and irreversible covalent PROTACs.  Journal of the American Chemical Society. 2020 Jul 8 , 142 (27):11734-11742.
  20. Giannoulis A., Feintuch A., Barak Y., Mazal H., Albeck S., Unger T., Yang F., Su X. & Goldfarb D. (2020). Two closed ATP- and ADP-dependent conformations in yeast Hsp90 chaperone detected by Mn(II) EPR spectroscopic techniques.  Proceedings of the National Academy of Sciences of the United States of America. 2020 Jan 7 , 117 (1):395-404.