Flow Cytometry About

Kleinman Cancer Cell Sorting Facility

Flow Cytometry is the measurement of single cells as they flow one by one past laser beams with the resulting light scatter and fluorescence emission detected and analyzed. Multiple biophysical properties of each individual cell can be measured at rates of thousands of cells per second. Cells are identified by their staining with fluorescent dyes that bind directly to specific constituents like DNA, RNA, proteins or ions or with dyes conjugated to molecules like antibodies, lectins, or hormones that bind to different cellular structures. Cells may also be identified following an infection or tranfection by marker fluorescent proteins. Flow Cytometry can detect and analyze multiple fluorescent staining simultaneously in each individual cell and can report and physically separate them by these qualities.

The Flow Cytometry unit contains six types of flow cytometers:

  1. Cell Analyzers - capable of cell analysis. The instruments available are: SORP-LSRII (LSRII-new) and ZE5, each with 5 lasers. LSRII-new can detect up to 17 colors, while ZE5 can detect up to 27 colors.
  2. Full spectrum Flow Cytometer- captures the full emission spectrum of every fluorescent molecule and enables characterization of up to 40 colors at a single cell level from each sample. The instrument available is Cytek™ Aurora cell analyzer with 5 lasers.
  3. Conventional Cell Sorters - can analyze and also sort objects ranging in size from 0.5 to 40 µm from chosen populations in a precise manner and deposit them into tubes or plates. This can be done under sterile conditions. The instruments available are: 2 FACSAriaIII with 5 lasers, SORP-FACSAriaII with 5 lasers, FACSymphony S6 with 5 lasers, FACSAriaIII with 4 lasers ,FACSAria FUSION with 4 lasers and BD FACSMelody with 3 lasers.
  4. Large Particle Sorter - The BioSorter is capable of analyzing and sorting objects ranging in size from 10 to 1,500 µm, aimed at sorting large and/or fragile cells. The instrument has 4 lasers and is capable of tracing fluorescence intensity changes across the whole length of the object.
  5. Imaging Cell Analyzer -capable of imaging cells in flow. The unit has 2 ImageStreamX Mark II analyzers with 4 lasers that can measure up to 12 parameters of bright-field and fluorescence and quantify staining intensity, pattern, localization and morphology.
  6. Mass Flow Cytometer - can analyze single cells, labeled with stable heavy metal isotopes using Mass Spectrometry. The "Helios" can resolve over 50 metal probes with minimal signal overlap.

The service provided incorporates assistance during planning, execution and analysis of experiments. The emphasis is on teaching multiple users to become independent.
Lab courses are conducted every year for students of the Institute.

Types of paid services available

Cell analysis - user operated.

Cell sorting - user operated.

Help per hour - work with close assistance of the staff.

This Unit operates within the Moross Integrated cancer center .

Micc, Moross Integrated Cancer Center