The Antibody Engineering Unit allows Weizmann’s scientists access to advanced protocols to preserve, generate and modify in-house antibodies.
Sequencing and cloning of antigen-specific antibodies from mouse germinal center B cells using single cell sorting and sequencing
Hybridomas secreting highly validated antibodies, take a significant time and resources to develop but are easily lost due to contamination, naturally occurring isotype switching or antibody production diminishes over time. Therefore, it is crucial to preserve valuable Antibody sequences following monoclonal antibody production or immediately upon receiving an outsourced Antibody producing hybridoma.
Hybridoma screening – to verify single clone origin
Production of a monoclonal antibody is often the key to its function, and any heterogeneity may influence our ability to establish the efficiency of the antibody. Therefore, it is important to verify monoclonality of each in-house or outsourced antibody producing hybridoma.
Switching Antibody Subtypes
In the unit, we can switch antibody subtypes from IgG2a/b/IgG3 to IgG1
Switch Antibody Species
In the unit, we can switch antibody species from murine to human (producing a chimeric antibody)
Switch Antibody Isotypes
In the unit, we can switch antibody isotypes from IgM to IgG
Reformat the Antibody
We offer to humanize the chimeric antibody via alignment with human germline sequences and CDR grafting
Large scale Antibody production
We use an adapted Expi293F expression system to produce large antibody quantities.