Flow cytometry safety

Safety Procedures and Guidelines

Laser safety

Safety form for Weizmann users
 

New supervisors as well as new users should fill the FACS safety form and give details about their project. The form may be accessed either after logging on to internal services or after entering the FACS reservation system.
While filling the form, special care should be taken to describe the cell types to be used as well as the biological or chemical risks involved in the project.
The form must be renewed every year, but should be modified every time a project changes.
After filling up the safety form, send an e-mail to the FACS staff with your full name and i.d. number in order to gain after hours access to the FACS Lab.
In case of malfunction of the entrance door off staff working hours do the following:

  1. Call 3515 or 2999
  2. In case of an emergency (like fire) if you are inside the lab, break the glass on the right side of the door
  3. Call the FACS staff.

Safety form for External users

External users should fill and submit the following biosafety form 

Biological safety

Working with cell sorters

The cell sorters create aerosols during their normal operation therefore special safety devices are used to minimize the risk of work with human material. The FACSAria, cell sorters have the "AMO" (Aerosol Management Option) systems. These are devices that promote the containment of aerosols by evacuating the air in the sort collection chamber, using vacuum and passing it through a filter. Users must learn to operate these systems before sorting with human cells. They should turn on the device and write down their name and the starting hour of the sort in the notebook. At the end of their work they have to turn it off and record ending hour.

Working with cell analyzers

The cell analyzers are closed systems without aerosols. However, waste fluids must be disposed off when the waste tanks are full. Add 500ml bleach to the empty waste tank of both LSR-II cell analyzers and 150ml bleach to the empty waste tank of the FACSort cell analyzer. When the waste tanks are full dispose of the liquid with the bleach in the sink.

General biosafety

  1. Users wear lab-coats and protective gloves wherever necessary.
  2. Smoking, eating or drinking are not permitted in the Lab.
  3. At the end of their work users must take the remains of their biological materials from the FACS room and dispose of it.

Laser safety

The lasers in this lab are classified as Class 4 and Class 3B laser products. A direct laser beam or mirror-like reflected beam of Class 4 has the potential to ignite flammable materials, to produce skin burn, and to cause severe eye damage. Exposure to a direct Class 3B laser beam or to a scattered Class 4 laser beam may also severely damage the eyes.

During regular work users are protected from a direct or reflected laser beam. Lasers are calibrated only by staff or outside technicians.

When a laser is calibrated check that;

  1. The warning light, in front of the sorter room entrance, is switched to "ON".

  2. The windows are fully covered.

  3. Everyone inside the lab is wearing a protective eyewear.

  4. For Class 4 only, wearing a white lab gown is recommended.

  5. Alignment of a visible beam should be done with the lowest possible power and with the use of alignment spectacles

  6. After completing the calibration switch the warning light to "OFF".

When an ignition of a flammable material occurs, use the extinguisher, located inside the lab, to extinguish the fire.

In case of a skin burn caused by a laser beam, cover the burn with a sterile wet pad and go to the local health clinic for additional treatment.

In case of an eye injury caused by a laser beam, cover the eye with a sterile wet pad and go to Kaplan Hospital for eye diagnosis and treatment.

Any laser incident should immediately be reported to the Laser Safety Officer.

When the red light is on in front of the sorters room users are not permitted to enter the room!!!!!!!