Our services include
- provide guidance and support throughout the various stages of the microbiome profiling process.
- Pre-consult, Form application, reports.
- Calibration for each research need including Academic, Industry and Governmental needs.
DNA Extraction from Environmental samples using the method/kit appropriate to the specific samples for PI and customer choices.
We offer two main kits for DNA Extraction: Zymo (ZR- D4308), and Omega (M5645-01) both can be used for manual and Automation extraction based on Tecan and Hamilton Robots.
16S/18S/ITS Amplicons (NGS)
Amplicon are PCR-amplified fragments from the DNA in duplicate using the high-fidelity Phusion Plus polymerase. A single round of PCR is done using primers (Illumina adaptors with indices and specific regions).
Targeting various sub-regions of interest, such as: 16S (Bacteria and Archaea), 18S (Eukarya, incl. Fungi) or ITS2 (Fungi only) genes.
Methods includes:
- Illumina library
- Home-made library
Shotgun metagenomics, is the untargeted ('shotgun') sequencing of all ('meta-') microbial genomes 'genomics' present in a sample. Shotgun sequencing can be used to profile taxonomic composition and functional potential of microbial communities and to recover whole genome sequences.
Preparation for amplicon or target-enrichment libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio® HiFi systems.