In case of Emergency call the following number 08-934-2999
The following guidelines are general and do NOT replace the specific safety guidelines described in the instructions manual of instruments, and on the WIS safety unit web site (see links bellow):
WIS Safety and Environment Unit general Regulations
WIS Biological Safety Regulations
WIS Chemical Safety Regulations
- Make sure you are familiar with general WIS emergency procedures.
- Identify the location of the nearest eyewash and shower and verify that they are accessible.
- Understand the hazards and toxicity of the materials you work with.
- All personnel should use basic laboratory protective gear: long trousers, closed shoes, and a lab-coat. Where needed additional protective equipment should be used (see below).
- Never operate an instrument that you did not use in the past without the proper training.
- When operating laboratory equipment, follow the safety signs (gloves, goggles, earphones), as well as the instruction card that is placed near the equipment.
- Do NOT use an instrument if you suspect is not functioning properly. Notify the personnel immediately!
- It is prohibited to eat and drink in the laboratory: you should use either the kitchen or the office for that.
- Wash your hands when you leave the lab, before eating or drinking, or have finished your daily work.
- At the end of the working day, please clean up your working environment, make sure the fridges doors are closed, close the door and turn off the lights.
Disposal of chemical waste
- Sequencing reagent bottles contain chemical waste that should be discarded as chemical waste in the chemical hood and labeled in accordance to the safety guidelines.
- The Shimadzu Protein sequencer uses the following reagents:
- Trimethlamine- Flammable liquid, Category 1, H224 Acute toxicity, Category 4, Oral, H302 Skin corrosion, Category 1B, H314 Specific target organ toxicity - single exposure, Category 3, Respiratory system, H335
- Ethyl Acetate- Highly flammable liquid and vapor Causes serious eye irritation May cause drowsiness and dizziness Prolonged or repeated contact may dry skin and cause irritation or cracking
- Trifluoroacetic Acid- May be corrosive to metals Causes severe skin burns and eye damage May cause respiratory irritation
- 1-chlorobutane- Highly flammable liquid and vapor May be fatal if swallowed and enters airways
- Phenyl Isothyocynate (5%)- Combustible liquid Causes severe skin burns and eye damage May cause respiratory irritation May cause an allergic skin reaction May cause allergy or asthma symptoms or breathing difficulties if inhaled Suspected of damaging the unborn child Toxic if swallowed, in contact with skin or if inhaled
- Acetonitrile- Potential Acute Health Effects: Hazardous in case of skin contact (irritant), of eye contact (irritant), of ingestion, of inhalation. Slightly hazardous in case of skin contact (permeator). DEVELOPMENTAL TOXICITY: Classified Reproductive system/toxin/female, Reproductive system/toxin/male [SUSPECTED].
- All above reagents must NEVER be discharged to the sanitary sewer in ANY concentration. These wastes must be collected and managed as chemical waste.
- When clearing the chemical waste, ensure the chemical hood is properly turned on, use protective wear and keep all containers in the hood tightly closed when not in use.
- Wash hands thoroughly each time gloves are removed.
- Remove waste containers to proper location when full.